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Dedifferentiating initiation and embryogenesis from freshly-isolated microspores of barley

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Abstract

By using DNA-specific fluorescent dye and a confocal laser scanning microscope, the present study was designed to investigate the cytological characteristics of dedifferentiating initiation during pretreatment and embryogenesis during culture in freshly-isolated microspores of barley, and the difference in main developmental pathway between freshly-isolated and cold-treated microspores. The results revealed that (i) freshly-isolated microspores started the initiation within 12 h of mannitol pretreatment, whose main cytological characteristics were that: cell volume was obviously extended; the volume of nuclei and nucleoli were also greatly increased; nucleoli were extremely clear and highly condensed; N/C ratio was very high; (ii) all the pretreatment methods led to the initiation of the microspores, thus triggering the embryogenic process; (iii) pretreatment methods influenced the main developmental pathway of microspores by changing the pattern of the first mitosis. The cold-treated microspores formed main developmental pathway via A patterns, but freshly-isolated microspores via B pattern.

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Authors and Affiliations

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics, Chinese Academy of Sciences, 100101, Beijing, China

    Guo Xiangrong, Jing Jiankang & Hu Han

Authors
  1. Guo Xiangrong
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  2. Jing Jiankang
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  3. Hu Han
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Project supported by the Bio-Rad China Limited.

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Guo, X., Jing, J. & Hu, H. Dedifferentiating initiation and embryogenesis from freshly-isolated microspores of barley. Sci. China Ser. C.-Life Sci. 40, 332–336 (1997). https://doi.org/10.1007/BF02879095

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  • DOI: https://doi.org/10.1007/BF02879095

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