Abstract
Protoplasts isolated from cell suspension cultures of potato were cultured as far as the level of regeneration of substrains. The influence of various factors (age of the cell culture, mode of precultivation, composition of the enzymatic mixture, incubation period) on the efficiency of isolation as well as the viability of protoplasts, has been studied. Various cultivation procedures have been screened and the effect of especially the hormonal composition of the medium on different phases of the regeneration of cell colonies (regeneration of the cell wall, growth and elongation, cell division) evaluated.
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Opatrný, Z., Schumann, U., Rakouský, S. et al. The role of some exogenous and endogenous factors in the isolation of protoplasts from potato cell cultures and their recovery in cell colonies. Biol Plant 22, 107–116 (1980). https://doi.org/10.1007/BF02878248
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DOI: https://doi.org/10.1007/BF02878248