Abstract
Electrophoretic separations of soluble proteins extracted from crown-gall tumors, normal calli, and leavesof Nicotiana plumbaginifolia were performed in order to detect pathogenic changes caused byAgrobacterium tumefaciens transformation. These analyses showed the appearance of new low-molecular polypeptides both in crown-gall tumors and in normal calli. The SDS-polyacrylamide gel electrophoresis as well as the gel electrophoresis in non-denaturing conditions were used to characterize their properties. At least some of them corresponded to the pathogenesis-related proteins reported inN. tabacum. Isoenzyme patterns of peroxidases and esterases also revealed a higher number and a deeper staining density of isoenzyrnes in crown-gall clones and normal calli compared with leaves, thus indicating an increased activity of enzymes in thesein vitro cultivated tissues.
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Vyskot, B., Kuhrová, V. Soluble proteins inNicotiana plumbaginifolia crown-gall tumors. Biol Plant 29, 143–149 (1987). https://doi.org/10.1007/BF02878169
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DOI: https://doi.org/10.1007/BF02878169