Abstract
We have found that the unorganized tobacco crown-gall tumor, isolated in our laboratory, and Braun's teratomatic tumor both exhibit the capacity to grow in the presence of high toxic concentrations of BUdR (up to 10-3 mol·l-1). Double cytokinin-auxin habituated tissues also exhibit a constitutive BUdR-tolerance. In cytokinin-habituated tissues the BUdR-tolerance seems to depend on the degree of cytokinin autonomy. The presence of exogenous cytokinin significantly increases BUdR-tolerance, and the presence of BUdR suppresses the inhibitory effect of exogenous cytokinin upon habituated tissues. The results clearly show that the disposition of a cell for transient BUdR tolerance requires at least active expression of genes for cytokinin synthesis and that the tolerance becomes permanent when the auxin system is turned on. We conclude that BUdR-tolerance is connected with the establishment of a certain hormonal regulator pattern, depending on the state of cellular differentiation.
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Bezděk, M., Reich, J., Tkadleček, L.: Properties of the cured oncogenic strain 37 400 of Agrobacterium tumefaciens. Folia Microbiol. 21, 371–377 (1976)
Bezděk, M., Vyskot, B., Koudelka, M.: The substitution of 5-bromodeoxyuridine for thymidine in the DNA of 5-bromodeoxyuridine tolerant tobacco tumor cells. Studia biophys. (1978) in press
Bezděk, M., Vyskot, B., Tkadlećek, L., Karpfel, Z.: Spontaneous high-level BUdR tolerance of the unorganized crown-gall tumor. Biochem. Physiol. Pflanzen 171, 437–446 (1977)
Binns, A., Meins, F., Jr.: Habituation of tobacco pith cells for factors promoting cell division is heritable and potentially reversible. Proc. Natl. Acad. Sci. (USA) 70, 2660–2662 (1973)
Braun, A.C.: A demonstration of the recovery of the crown-gall tumor cell with the use of complex tumors of single-cell origin. Proc. Natl. Acad. Sci. (USA) 45, 932–938 (1959)
Kiminek, M., Luštinec, J.: Induction of cytokinin-autonomy and chlorophyll-deficiency in tobacco callus tissue by streptomycin. Z. Pflanzenphysiol. 73, 74–81 (1974)
Kandra, G., Maliga, P.: Is BUdR-resistance a consequence of cytokinin-habituation in Nicotiana tabacum? Planta 133, 131–134 (1977)
Limasset, P., Gautheret, R.: Sur le caractère tumoral des tissus de tabac ayant subi le phénomène d'accoutumance aux hétéroauxines. C.R. Acad. Sci. Paris. 230 D, 2043–2045 (1950)
Luk, D.C., Bick, M.D.: Determination of 5′-bromodeoxyuridine in DNA by buoyant density. Anal. Biochem. 77, 346–349 (1977)
Márton, L., Maliga, P.: Control of resistance in tobacco cells to 5-bromodeoxyuridine by a simple mendelian factor. Plant. Sci. Lett. 5, 77–81 (1975)
Meins, F., Jr.: 5-Bromodeoxyuridine: a specific inhibitor of cytokinin-habituation in tobacco cell culture. Planta 129, 239–244 (1976)
Murashige, T., Skoog, F.: A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiol. Plant. 15, 473–497 (1962)
Opatrný, Z., Opatrná, J.: The specificity of the effect of 2,4-D and NAA on the growth, micromorphology, and occurrence of starch in long-term Nicotiana tabacum L. cell strains. Biol. Plant. 18, 359–365 (1976)
Syōno, K., Furuya, T.: Effects of cytokinins on the auxin requirement and auxin content of tobacco calluses. Plant & Cell Physiol. 13, 843–856 (1972)
Syōno, K., Furuya, T.: Induction of auxin-nonrequiring tobacco calluses and its reversal by treatments with auxins. Plant & Cell Physiol. 15, 7–17 (1974)
Vyskot, B., Bezděk, M., Karpfel, Z.: Induction and culture of crown-gall tumors of haploid tobacco in vitro. Biochem. Physiol. Pflanzen 170, 417–426 (1976)
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Vyskot, B., Karpfel, Z. & Bezděk, M. Hormonal control of 5-bromodeoxyuridine (BUdR) tolerance in crown-gall tumors and habituated tissues. Planta 137, 247–252 (1977). https://doi.org/10.1007/BF00388158
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DOI: https://doi.org/10.1007/BF00388158