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The effect of indole-3-Acetic acid on ethylene formation in wheat seedlings

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Isoperoxidase B 1 isolated from winter wheat (Triticum aestivum L., cv. Jubilar) seedlings was shown to catalyze ethylene formation from α-keto, γ-methylmercaptobutyric acid (KMBA). In the presence of Mn2+, indole-3-acetic acid (IAA), andp-coumaric acid, the kinetics by isoperoxidase B 1 catalyzed conversion of KMBA into ethylene and other products was similar to that of IAA oxidation. The reaction rate was therefore controlled by IAA through its electrondonating properties.

Exogenous IAA induced ethylene formation in the segments of etiolated wheat coleoptiles. IAA-induced ethylene production was enhanced by L-methionine and mitomycin C. Aminoethoxy-analogue of rhizobitoxine, ferulic acid, sodium benzoate, cycloheximide and actinomyoin D exhibited significant inhibitory effects. These data indicate that the overall reaction mechanism in coleoptile segments involves RNA and protein synthesis.

The site of IAA action is not specific; 2,4-dichlorophenoxyacetic, α-naphthylacetic and indole-3-butyric acids, respectively, possessed comparable inductive effect as IAA. Indole-3-propionic acid, indole, L-tryptophan and glucobrassicin had only low inductive efficiency, and moreover indole and L-tryptophan slowed down IAA-induced ethylene formation.

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Abbreviations

IAA:

indole-3-acetic acid

KMBA:

α-keto, γ mothylmercaptobutyric acid

HOA:

4-hydroxycinnamic(p-coumaric) acid

PA:

3-mothcxy, 4-hydroxycinnamic (ferulic) acid

2,4-D:

2,4-dichlorophencxyacetic acid

NAA:

α-naphthylacetic acid

IBA:

indole-3-bubyric acid

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Author notes

  1. V. Našinec

    Present address: Institute of Experimental Botany, Czechoslovak Academy of Sciences, Praha, Ke dvoru 16, 16630, Praha 6, Czechoslovakia

Authors and Affiliations

  1. Department of Plant Nutrition, Research Institute of Crop Production, Drnovská 507, 16106, Praha 6, Czechoslovakia

    Ivana Macháčková, V. Našinec & Z. Zmrhal

Authors
  1. Ivana Macháčková
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  2. V. Našinec
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  3. Z. Zmrhal
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Macháčková, I., Našinec, V. & Zmrhal, Z. The effect of indole-3-Acetic acid on ethylene formation in wheat seedlings. Biol Plant 22, 65–72 (1980). https://doi.org/10.1007/BF02878130

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  • DOI: https://doi.org/10.1007/BF02878130

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