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The use of vancomycin for the isolation of auxotrophic mutants inMycobacterium smegmatis

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Abstract

A method using vancomycin for the accumulation of auxotrophic mutants ofMycobacterium smegmatis M54/81 induced by N-methyl-N′-nitro-N-nitrosoguanidine was developed. As compared with the simple replication technique the yield of auxotrophic mutants was twenty-fold.

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    J. Koníček, M. Koníčková-Radochová & V. Rytíř

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  1. J. Koníček
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  2. M. Koníčková-Radochová
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  3. V. Rytíř
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Koníček, J., Koníčková-Radochová, M. & Rytíř, V. The use of vancomycin for the isolation of auxotrophic mutants inMycobacterium smegmatis . Folia Microbiol 28, 134–137 (1983). https://doi.org/10.1007/BF02877370

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  • DOI: https://doi.org/10.1007/BF02877370

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