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Occurrence of alkali-labile sites in pulse labelled DNA ofEscherichia coli after the action of DNA-damaging chemicals

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Abstract

UV irradiatedE. coli cells contain alkali labile sites in their DNA daughter chains. It is shown here that the occurrence of alkali labile sites in the newly synthesized DNA is a more common phenomenon. It occurred after treatment with two different DNA damaging chemicals, but not after treatment with nalidixic acid.

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References

  • Cozzarelli N.R.: DNA gyrase and the supercoiling DNA.Science 207, 953–960 (1980).

    Article  PubMed  CAS  Google Scholar 

  • Iyeb V.N., Szybalski W.: A molecular mechanism of mitomycin action: linking of complementary DNA strand.Proc.Nat.Acad.Sci. 50, 355–362 (1963).

    Article  Google Scholar 

  • Iyer V.N., Szybalski W.: Mitomycin and porfiromycin: chemical mechanism of action and cross linking of DNA.Science 145, 55–58 (1964).

    Article  PubMed  CAS  Google Scholar 

  • Sedliaková M., Slameňová D., Štukovský R.: Changes in the radioresistance of bacteria after the inhibition of proteosynthesis in the preirradiation phase.Folia Microbiol. 11, 169–172 (1966).

    Article  Google Scholar 

  • Slezáriková V., Sedliaková M.: The presence of alkali labile sites in DNA daughter chains of UV irradiatedE. coli. FEBS Letters148, 251–254 (1982).

    Article  PubMed  Google Scholar 

  • Slezáriková V., Sedliaková M.: Occurrence and loss of alkali labile sites in newly synthesized DNA of UV irradiatedEscherichia coli K12.Mutat.Res. 132, 79–86 (1984).

    PubMed  Google Scholar 

  • Sugino A., Peebles C.L., Kreuzer K.N., Cozzabelli N.R.: Mechanism of action of nalidixic acid. Purification ofE.coli nalA gene product and its relationship to DNA gyrase, a novel nicking closing enzyme.Proc.Nat.Acad.Sci. 74, 4767–4771 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Velemínský J., Gichner T.: Interaction of alkylating agents with DNA and their significance for the formation of gene mutation.Biol. listy 47, 33–58 (1982).

    Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Genetics, Cancer Research Institute, Slovak Academy of Sciences, 812 32, Bratislava, Czechoslovakia

    V. Slezáriková & M. Sedliaková

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  1. V. Slezáriková
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  2. M. Sedliaková
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Slezáriková, V., Sedliaková, M. Occurrence of alkali-labile sites in pulse labelled DNA ofEscherichia coli after the action of DNA-damaging chemicals. Folia Microbiol 32, 531–534 (1987). https://doi.org/10.1007/BF02877202

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  • DOI: https://doi.org/10.1007/BF02877202

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