Abstract
The localization of the formic dehydrogenase system was studied by means of nitro-blue tetrazolium, using the ultrathin section technique, in large long bodies ofProteus vulgaris, which form the intermediate stage in regeneration of penicillin-induced spheroplasts. In the electron microscope, empty areas, numbering on an average four per cell section, were found at the site of formazan deposits, while in the controls (without tetrazolium salts) the average number was 0.05. From these findings, and from the findings of other authors showing that formic dehydrogenase is bound to cell structures, it can be concluded that inProteus cells this enzyme is bound to the cytoplasmic membrane.
Abstract
Локализация системы дегидрогеназы муравьиной кислоты исследовалась с применением nitro-blue-тетразолияитехники ультратонких срезов. Объектом исследования являлись крупные продолговатые тельца Proteus vulgaris, представляющие собой переходную стадию в процессе регенерации пенициллиновых сферопластов. В месте расположения формазана под электронным микроскопом были обнаружены пустоты, средним числом по 4 на 1 срез через клетку, тогда как в контроле (без соли тетразолия) их приходилось только 0,05 на 1 срез. На основании зтих наблюдений, а также данных других авторов, что дегидрогеназа муравьиной кислоты связывается с клеточными структурами, можно полагать, что в клетках протея этот фермент связан с цитоплазматической оболочкой.
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Nermut, M.V., Rýc, M. Electron microscopy of the localization of formic dehydrogenase in regenerating forms ofProteus vulgaris . Folia Microbiol 9, 16–20 (1964). https://doi.org/10.1007/BF02875895
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DOI: https://doi.org/10.1007/BF02875895