Skip to main content
SpringerLink
Log in

The antigenic structure ofFrancisella tularensis

I. Isolation of the ether antigen and its fractionation

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The ether antigen ofFrancisella tularensis was submitted to fractionation using ammonium sulphate, ethanol and trichloracetic acid (TCA). A simple antigenic mixture was obtained by this fractionation from the original complex ether antigen. However, no separation of antigenically and chemically homogenous substances was achieved, by this procedure. The precipitation with TCA permitted the separation of an antigenic component that was found to be identical with the phenol antigen or its component migrating faster towards cathode in electrophoresis. The sediment obtained possessed common properties with the precipitate obtained by ethanol precipitation. The fraction having the highest anodic mobility could be obtained by salting out the original antigen with ammonium sulphate to 50% saturation. By increasing the concentration of ammonium sulphate to 60% saturation, all components of the ether antigen could be precipitated. The number of precipitation lines in the original antigen and its fractions depends both on the concentration of antigen and the quality of antisera.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aleshina E. N., Puchkova T. I.: The fibrinolytic properties of tularemy microbe. (In Russian)Trudy Rostovskogo na Donu Ghos. Nauchno-Issled. Protivochumn. Inst. 6, 96 (1947).

    Google Scholar 

  • Berenblum I., Chain E.: An improved method for the colorimetric determination of phosphate.Biochem. J. 32, 295 (1938).

    PubMed  CAS  Google Scholar 

  • Boivin A., Mesrobeanu L.: Contribution á l'étude de la composition chimique de bactéries. Substances azotées et phosphorées “acido-solubles”.Compt. Rend. Soc. Biol. 112, 76 (1933).

    CAS  Google Scholar 

  • Dische Z. Über Mikrobestimmung der Kohlenhydrate in tierischen Organen und im Blute mit Hilfe charakteristischer Farbreaktionen.Mikrochemie 7, 33 (1929).

    Article  CAS  Google Scholar 

  • Francis E., Evans A. Agglutination, cross-agglutination and agglutination absorption in tularemis.Publ. Health Rep. 41, 1273 (1926).

    Google Scholar 

  • Galperin M. E.: The striction method for detection of toxins in infectious diseases.Autoref., Diss. Thesis, Moscow 1955.

  • Grabar, P., Burtin P.: Immunoelectrophoretic Analysis. p. 39 Elsevier Publ. Co., Amsterdam 1964.

    Google Scholar 

  • Kalacheva A. G., Kleush-Ivanova Z. P., Kratinova P. N.: The effect of tularemia toxin on the striction reaction of tissues. (In Russian)Zh. Microbiol Epidemiol. Immunol. 1; 77 (1948).

    Google Scholar 

  • Kalacheva N. F.: Serological affinity of strains of tularemia and the differencies of tularemy and brucella microbes. (In Russian)Tr. Inst. “Microbiol”, Sarator1, 115 (1951).

    Google Scholar 

  • Lowry O. H., Roseborough N. J., Farr A. L., Randall R. J.: Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265 (1951).

    PubMed  CAS  Google Scholar 

  • Ormsbee R. A., Bell J. F., Larson C. L.: Studies ofBacterium tularense antigens. I. The isolation, purification and biologic activity of antigen preparations fromBacterium tularense.J. Immunol. 74, 351 (1955).

    PubMed  CAS  Google Scholar 

  • Ormsbee R. A., Larson C. L.: Studies onBacterium tularense antigens. II. Chemical and physical characteristics of protective antigen preparations.J. Immunol. 74, 359 (1955).

    PubMed  CAS  Google Scholar 

  • Owen C. R., Bell J. F., Larson C. L., Ormsbee R. A.: Virulence ofBacterium tularense. II. Evolution of criteria of virulence ofBacterium tularense.J. Inf. Diseases,97, 167 (1955).

    CAS  Google Scholar 

  • Óz T. V.: Endotoxin ofPasteurella tularensis.Türk. Ztschrf. Hyg. Exp. Biol. II, 187 (1940).

    Google Scholar 

  • Shipitsina C. K. Chemical composition of antigenic material of tularemia.Proc. Acad. Sci USSR 105, 315 (1955).

    Google Scholar 

  • Shipitsina C. K.: The correlation of chemical structure of specific material of tularemy, microbe and its virulence. Thesis Conf.: Variability of microorganisms and bacteriophagy. p. 63 (1958).

  • Skrodzki E.: Characteristic ofB. tularense strains cultivated in Poland in the years 1952–53.Med. Doświad. Mikrobiol. 1, 65 (1956).

    Google Scholar 

  • Škvařil F.: The micromodification of immunoelectrophoresis. (In Czech)Chem. listy 55, 1069 (1961).

    Google Scholar 

  • Tinker I. S., Drozhevkina M. S., Konnova A. M., Grzhebina N. K. The whole antigen of tularemy microbe.Sborn. Tr. Astrakhan. Protivochumn. Stantsii 1, 135 (1955).

    Google Scholar 

  • Vereninova N. K. The diffusion factor of tularemy microbe. (In Russian)Tr. Inst. “Mikrob”, Saratov1, 39 (1951).

    Google Scholar 

  • Vereninova N. K., Kontorina, A. A., Bakhrakh Ye. E.: The characteristic of polysaccharide-containing fractions of tularemy microbe. (In Russian)Zh. Mikrobiol. Epidemiol. Immunol. 2, 8 (1956).

    Google Scholar 

  • Yemelyanova O. S.: Some problems of microbiology of tularemia. (In Russian)Zh. Mikrobiol. Epidemiol. Immunol. 11, 10 (1946).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Military Institute of Hygiene, Epidemiology and Microbiology, Prague 6

    O. Procházka & H. Dubanská

Authors
  1. O. Procházka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Dubanská
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Procházka, O., Dubanská, H. The antigenic structure ofFrancisella tularensis . Folia Microbiol 17, 232–238 (1972). https://doi.org/10.1007/BF02875819

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02875819

Keywords

Search

Navigation