Abstract
The Yersinia entomophaga toxin complex (Yen-TC) is the bacterium’s main virulence determinant. Because of its high insect activity, methods were developed to allow the routine isolation and purification of Yen-TC from an overnight bacterial culture using size exclusion chromatography. Here we outline an overnight purification procedure using a 100-ml culture volume, where approximately 2 mg of Yen-TC, with an approximate purity of 95–98 %, can be routinely obtained.
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Acknowledgement
This work was supported by the New Zealand Foundation for Research, Science, and Technology (C10X0804).
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Jones, S.A., Hurst, M.R.H. (2016). Purification of the Yersinia entomophaga Yen-TC Toxin Complex Using Size Exclusion Chromatography. In: Glare, T., Moran-Diez, M. (eds) Microbial-Based Biopesticides. Methods in Molecular Biology, vol 1477. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6367-6_4
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DOI: https://doi.org/10.1007/978-1-4939-6367-6_4
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6367-6
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