Abstract
The bactericidal activity of sera not containing antibodies (sera from precolostral piglets and calves) was tested with strains of gram-negative bacteria with different surfaces. The accuracy of the method of bactericidal test was evaluated statistically the bactericidal unit of complement was defined for comparing the activity of sera of different animals and different species. Various methods used for estimating the character of bacterial surface were compared.
It was found that the bactericidal activity of piglet sera is directly dependent on the content of complement in the sera tested and the character of the bacterial surface (in the R-form). In selected strains there is a correlation in all criteria characterizing the surfaces of bacteria, and their susceptibility to bactericidal activity of sera; in a group of 37 strains selected at random, correlation with only one of the surface characteristics (stability in solution after heating to 100°C for 1 hour) was found.
In calf sera a component was found which increases the effect of complement to some strains (e.g.Shigella shigae). This component may by absorbed from the serum only in the presence of complement. The nature of this factor is discussed.
Abstract
Изучалось бактерицидное действие сывороток, не содержащих антител (сывороток от преколостральных поросят и телят, еще не получавших молозива), на штаммы грамотрицательных бактерий с различным характером поверхности. Точность методики пробы бактерицидности определяли статистическим методом. Для сравнения эффективности сывороток различных животных и различных видов была определена единица бактерицидности комплемента. Сравнивались различные методы определения характера поверхности бактерий.
Было установлено, что существует прямая зависимость между бактерицидным действием сывороток поросят и содержанием комплемента в этих сыворотках и характером поверхности микробов (Я-фазой). У избранных штаммов имеет место корреляция со всеми показателями, характеризующими поверхность микробов и чувствительностью этих микробав к бактерицидному действию сывороток; у случайно подобранной группы 37 штаммов имела место корреляция только с одним показателем (стабильность бакт. суспензии после прогревания в течение часа при 100°C).
В телячьих сыворотках был обнаружен компонент, который усиливает действие комплемента на некоторые штаммы (напр., Shigella shigae). Этот компонент можно извлечь из сыворотки истощением только в присутствии комплемента. —Обсуждается характер этого фактора.
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Šterzl, J., Pešák, V., Kostka, J. et al. The relation between the bactericidal activity of complement and the character of the bacterial surfaces. Folia Microbiol 9, 284–298 (1964). https://doi.org/10.1007/BF02873307
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DOI: https://doi.org/10.1007/BF02873307