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Action of leptospiral lipases on purified serum lipoproteins

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Abstract

Partially purified exocellular lipases produced by a pathogenic (Leptospira copenhageni (PB-3) and a saprophytic strain ofLeptospirae (Leptospira Patoc I) hydrolyzed very low density, and to a lesser extent low density hog serum lipoproteins. No high density lipoprotein hydrolysis was found. Optimal conditions for the action of both lipases on very low density serum lipoproteins were determined: pH 8.5; NaCl 0.4m; CaCl2 1.0mm; sodium desoxycholate 10.60mm. The maximal velocities of very low density lipoprotein hydrolysis were compared with the maximal velocities of triglyceride emulsion hydrolysis by both lipases.

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Authors and Affiliations

  1. Department of Epidemiology, Medical Faculty, Comenius University Bratislava, Gainesville

    B. Chorváth & M. Fried

  2. Department of Biological Chemistry, College of Medicine, University of Florida, Gainesville

    B. Chorváth & M. Fried

Authors
  1. B. Chorváth
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  2. M. Fried
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Chorváth, B., Fried, M. Action of leptospiral lipases on purified serum lipoproteins. Folia Microbiol 15, 303–308 (1970). https://doi.org/10.1007/BF02869058

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  • DOI: https://doi.org/10.1007/BF02869058

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