Abstract
Auxotrophic mutants ofCorynebacterium sp. VÚA 9366 used for industrial biosynthesis of some amino acids were induced by UV-light and nitrogen mustard. The mutants were isolated by means of the penicillin technique. It was assumed when comparing mutation spectra after the treatment with both mutagens that the mutant cell gives origin to a clone of genetically identical cells; it was hence possible to express numbers of mutants as corresponding numbers of mutations. Requirements for vitamins, components of nucleic acids and amino acids were studied. It was found that mutations causing requirements for arginine which can be substituted by citrulline or ornithine, requirement for valineisoleucine and components of nucleic acids are significantly more frequent after treatment with UV-light. On the other hand, mutations leading to the requirement for arginine which can be substituted by citrulline were more frequent after treatment with nitrogen mustard.
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Nečásek, J., Plachý, J. The relative specificity of mutagens for the induction of forward mutations inCorynebacterium . Folia Microbiol 15, 275–281 (1970). https://doi.org/10.1007/BF02869054
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DOI: https://doi.org/10.1007/BF02869054