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Modified method for determination of serum beta-glucuronidase: A comparative study using P-nitrophenyl glucuronide and phenolphthalein glucuronide as substrate in gastrointestinal tract carcinomas

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Abstract

Serum beta-glucuronidase activity was estimated by our modified method using two substrates phenolphthalein glucuronide and p-nitrophenyl glucoronide in 49 healthy subjects and 94 patients with gastrointestinal tract (GIT) carcinoma. The correlation coefficient using the two substrates was found to be r=0.8383. The method of Gabor Szasz was modified wherein the incubation time was decreased from 5 hours to 2 hours and the incubation temperature was increased from 25°C to 37°C. The increase in beta-glucuronidase activity in GIT carcinoma appears to be a good biochemical marker in patients with such type of carcinomas, a finding not reported in literature to-date.

The modified quantitative method used for estimation of beta-glucuronidase is reliable, accurate, simple and rapid.

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Authors and Affiliations

  1. Department of Biochemistry, Tata Memorial Hospital, B/2, Gnyandeep CHS, 17th Road, Santacruz (West), 400 012, Mumbai, India

    M. M. Khanolkar, A. V. Sirsat & S. S. Walvekar

  2. Department of Surgery, Tata Memorial Hospital, Parel, 400 012, Mumbai, India

    M. S. Bhansali & L. J. Desouza

Authors
  1. M. M. Khanolkar
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  2. A. V. Sirsat
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  3. S. S. Walvekar
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  4. M. S. Bhansali
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  5. L. J. Desouza
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Khanolkar, M.M., Sirsat, A.V., Walvekar, S.S. et al. Modified method for determination of serum beta-glucuronidase: A comparative study using P-nitrophenyl glucuronide and phenolphthalein glucuronide as substrate in gastrointestinal tract carcinomas. Indian J Clin Biochem 12, 67–70 (1997). https://doi.org/10.1007/BF02867959

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  • DOI: https://doi.org/10.1007/BF02867959

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