Abstract
Low density lipoprotein (LDL) was isolated from normal rabbits and was modified with acetic anhydride. Blood monocyte derived macrophages from normal and hypercholesterolemic (HC) rabbits were cultured, and on the 8th day the cells were incubated with native and modified LDL to study their binding and degradation. Macrophages from both normal and hypercholesterolemic rabbits express a limited number of receptors for normal plasma LDL whereas they exhibit a large number of receptors for acetyl LDL. There was no significant difference between binding and degradation of acetyl LDL by normal or hypercholesterolemic cells. However, binding and degradation of native LDL by monocytes of hypercholesterolemic cells were significantly less (p<0.0001) in comparison to binding and degradation of native LDL by normal cells indicating that there is a feedback inhibitory pathway in the cell that inhibits the synthesis of LDL receptors in the presence of excess LDL.
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References
Faggiotto, A., Ross, R. and Harker, L. (1984) Studies on hypercholesterolemia in the non human primates: Changes that lead to fatty streak formation. Arteriosclerosis. 4, 323–340.
Fowler, S., Shio, H. and Haley, N.J. (1979) Characterization of lipid laden aortic cells from cholesterol fed rabbits. IV Investigation of macrophage like properties of aortic cell populations. Lab. Invest. 41, 372–378.
Gerrity, R.G., Naito, H.K., Richardson, M. and Schwartz, C.J. (1979) Dietary induced atherogenesis in swine. Morphology on the intima in prelesion stages. Am. J. Pathol. 95, 775–792.
Gerrity, R.G. (1981) The role of the monocyte in atherogenesis. I. Transition of blood borne monocytes into foam cells in fatty acid lesions. Am. J. Pathol. 103, 181–190.
Aquel, N.M., Ball, R.Y., Waldman, H. and Mitchinson, K.J. (1984) Monocytic origin of foam cells in human atherosclerotic plaques. Atherosclerosis. 53, 265–271.
Jerome, W.G. and Lewis, J.C. (1985) Early atherogenesis in white cameau pigeons. II. Ultrastructural and cytochemical observations. Am. J. Pathol. 119, 210–222.
Lewis, J.C., Taylor, R.G. and Jerome, W.G. (1985) Foam cell characteristics in coronary arteries and aortas of white carneau pigeons with moderate hypercholesterolemia. Ann. N.Y. Acad. Sci, 454, 91–100.
Achtani, C., Awtade, A., Vasisht, S. and Srivastava, L.M. (1995) Effect of MaxEPA (Fish oil) on lipoproteins and its receptors in hypercholesterolemic rabbits. Biochem. Mol. Biol. Int. 37, 489–498.
Goldstein, J.L., Ho, H.K.J., Basu, S.K. and Brown, M.S. (1979) Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition. Proc. Natl. Acad. Sci. USA, 76, 333–337.
Basu, S.K., Brown, M.S., Ho, Y.K. and Goldstein, J.L. (1979) Degradation of low density lipoprotein, dextran sulfate complexes associated with deposition of cholesterol esters in mouse macrophages. J. Biol. Chem. 254, 7141–7146.
Goldstein, J.L., Ho, Y.K., Brown, M.S., Innerarity, T.L. and Mahley, R.W. (1980) Cholesteryl ester accumulation in macrophages resulting from receptor mediated uptake and degradation of hypercholesterolemic canine beta very low density lipoproteins. J. Biol. Chem. 255, 1839–1848.
Steinberg, D. and Witztum, J.L. (1990) Lipoproteins and atherogenesis. J. Amer. Med. Assoc. 264, 3047–3052.
Das, S., Snehlata and Srivastava, L.L. (1997) Effect of ascorbic acid on lipid profile and lipid peroxidation in hypercholesterolemic rabbits. Nutr. Res. 17, 231–241.
Jialal, I. and Devaraj, S. (1996) The role of oxidized low density lipoprotein in atherogenesis. J. Nutr. 126, 1053S-1057S.
Havel, R.J., Eder, H.A. and Bragdon, J.H. (1955) The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J. Clin. Invest. 34, 1345–1353.
Basu, S.K., Goldstein, J.L., Richard, G.W., Anderson and Brown, M.S. (1976) Degradation of cationized low density lipoprotein and regulation of cholesterol metabolism in homozygous familial hypercholesterolemia fibroblasts. Proc. Natl. Acad. Sci. USA, 73, 3178–3182.
Van, T.A., Van, G.T. and Vant, H.F. (1978) High density lipoprotein catabolism before and after partial hepatectomy. Atherosclerosis. 29, 439–448.
Current, protocols in immunology, Suppl. 13, John Wiley & Sons Inc., New York, Chapter 7, p7.1.1.
Goldstein, J.L., Basu, S.K., Brunschede, G.Y. and Brown, M.S. (1976) Release of low density lipoprotein from its cell surface receptor by sulfated glycosaminoglycans. Cell. 7, 85–95.
Johnson, W.D., Mei, B. and Cohn, Z.A. (1977) The separation, long term cultivation and maturation of the human monocytes. J. Exp. Med. 146, 1613–1626.
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Das, S., Snehlata & Srivastava, L.M. Binding and degradation of native and acetylated low density lipoproteins by monocyte derived macrophages of normal and hypercholesterolemic rabbits. Indian J Clin Biochem 12, 59–62 (1997). https://doi.org/10.1007/BF02867957
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DOI: https://doi.org/10.1007/BF02867957