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A Low-Molecular-Weight Phenotype of Apolipoprotein(a) as a Factor Provoking Accumulation of Cholesterol by THP-1 Monocyte-Like Cells

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Increased concentration of lipoprotein(a) is a risk factor of coronary heart disease. lipoprotein(a) consists of LDL-like and highly polymorphic apolipoprotein(a). Here we studied the effect of lipoprotein(a)-containing sera with different apolipoprotein(a) phenotypes on lipid accumulation by THP-1 monocyte-like cells. Cholesterol concentration in lysates of THP-1 cells was significantly higher after their incubation with lipoprotein(a)-containing serum samples with low-molecular-weight phenotype of apolipoprotein(a) in comparison with samples with a high-molecular-weight apolipoprotein(a) phenotype irrespective of initial cholesterol level as well as serum concentrations of apoB-100, oxidized LDL, and circulating immune complexes. The presence of the most atherogenic small dense LDL subfractions in examined sera in addition to a low-molecular-weight apolipoprotein(a) phenotype resulted in significant elevation of cholesterol accumulation by THP-1 cells. The data obtained explain greater atherogenicity of lipoprotein(a) with low-molecular-weight apolipoprotein(a) phenotype.

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Authors and Affiliations

  1. National Medical Research Center of Cardiology, Ministry of Health of the Russia, Moscow, Russia

    O. I. Afanas’eva, E. B. Vikhrova, O. A. Razova, E. A. Utkina, M. I. Afanas’eva, E. A. Klesareva, N. V. Radyukhina, T. I. Aref’eva & S. N. Pokrovsky

Authors
  1. O. I. Afanas’eva
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  2. E. B. Vikhrova
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  3. O. A. Razova
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  4. E. A. Utkina
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  5. M. I. Afanas’eva
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  6. E. A. Klesareva
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  7. N. V. Radyukhina
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  8. T. I. Aref’eva
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  9. S. N. Pokrovsky
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Corresponding author

Correspondence to O. I. Afanas’eva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 1, pp. 27-32, January, 2019

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Afanas’eva, O.I., Vikhrova, E.B., Razova, O.A. et al. A Low-Molecular-Weight Phenotype of Apolipoprotein(a) as a Factor Provoking Accumulation of Cholesterol by THP-1 Monocyte-Like Cells. Bull Exp Biol Med 167, 24–29 (2019). https://doi.org/10.1007/s10517-019-04452-w

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  • DOI: https://doi.org/10.1007/s10517-019-04452-w

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