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Sensitivity and specificity of isolated antigen fromPlasmodium falciparum culture supernatant

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Abstract

Immunological sensitivity and specificity properties of isolated Plasmodium falciparum (GPL) antigen from culture supernatant have been measured and compared with malarial antigens and non malarial filtered paper blood sera for potency and efficacy. Latex bead coded GPL, Pf and RESA antigens immunoreaction properties of human filter paper blood samples (FPB) were studied by laser light scattering immunoassay (LIA) and Enzyme linked immunoabsorbent assay (ELISA) techniques. Results of GP. antigen sensitivity study by LIA method showed a very high malaria antibody binding response (MABR) i.e. 6% compared with 78% with RESA and 88% Pf antigens. Malaria detection by ELISA method also found similar results. Specificity study of GPL antigen for different non malarial filter paper blood sera (NMFS) showed no immunoreaction however Pf and RESA antigen showed few positive immunological responses. These results suggest that sensitivity and specificity properties of isolated GPL antigen is better than other antigens.

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Authors and Affiliations

  1. Centre for Biomedical Engineering, Indian Institute of Technology, 110016, New Delhi

    M. M. Mya & R. K. Saxena

  2. Malaria Research Centre, 22, Shamnath Marg, 110054, Delhi

    Arati Roy

Authors
  1. M. M. Mya
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  2. R. K. Saxena
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  3. Arati Roy
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Correspondence to R. K. Saxena.

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Mya, M.M., Saxena, R.K. & Roy, A. Sensitivity and specificity of isolated antigen fromPlasmodium falciparum culture supernatant. Indian J Clin Biochem 17, 75–82 (2002). https://doi.org/10.1007/BF02867946

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