Abstract
Disc eletrophoresis was employed to separate soluble proteins and esterase in mycelial extracts ofStreptomyces scabies, an acid tolerantStreptomyces sp. pathogenic on potatoes, and two nonpathogenicStreptomyces spp. Each organism demonstrated a characteristic pattern of major protein bands. Distinctive esterase patterns were also obtained, although one band of esterase activity was common to all organisms and most bands were shared by two or more organisms.
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Literature Cited
Bonde, M. R. 1967. Isolation and classification of the common scab organism. A Thesis. Univ. of Maine.
Bonde, M. R. and G. A. McIntyre. 1968. Isolation and biology of aStreptosvces sp. causing potato scab in soils below pH 5.0. Amer. Potato J. 45: 273–278.
Douglas, R. J. and E. H. Garrard. 1954. Serological observations on the actinomycetes associated with potato scab. Can. J. Bot. 32: 480–485.
Gomori, G. 1952. Microscopic histochemistry. Univ. of Chicago Press, Illinois. 273 pp.
Gottlieb, D. and Pamela M. Hepden. 1966 The electrophoretic movement of proteins from variousStreptomyces spp. as a taxonomic criterion. J. Gen. Microbiol. 44: 95–104.
Manzer, F. E. Unpublished data.
McCrum, R. C. and F. E. Manzer. 1967. Serological comparison ofStreptomyces spp. isolated from potato. Amer. Potato J. 44: 338.
Norris, J. R. 1969. The application of gel electrophoresis to the classification of microorganisms.In Chemotaxonomy and Serotaxonomy I-XVI.
Ornstein, L. and B. J. Davis. 1961. Disc electrophoresis. Preprint by Distillation Products Industries (Eastman Kodak Co.), New York. 66 pp.
Ornstein, L. 1964. Disc electrophoresis. I. Background and Theory. Ann. New York Acad. Sci. 121: 321–349.
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Hughes, C., McCrum, R.C. & Manzer, F.E. Protein and esterase electrophoretic patterns of streptomyces SPP. isolated from Maine potatoes. American Potato Journal 48, 206–213 (1971). https://doi.org/10.1007/BF02862996
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DOI: https://doi.org/10.1007/BF02862996