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Isolation of hepatic drug metabolism inhibitors from the seeds ofMyristica fragrans

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Abstract

The hexane extract from Nutmeg, the seed ofMyristica fragrans significantly inhibited hepatic drug-metabolizing enzyme activity. Through systematic fractionation by SiO2 column and vacuum liquid chromatography monitoring by bioassay, three components, myristicin, (I), licarin-B(II) and dehydrodiisoeugenol(III) were isolated as active principles. Compounds II and III, with a single treatment (200 mg/kg, i.p.) showed not only a significant prolongation of hexobarbital-induced sleeping time but also a significant inhibition of aminopyrine N-demethylase and hexobarbital hydroxylase activities in mice. Compounds I and II provoked a sleep episode at a subhypnotic dose of HB, suggesting that they posses CNS-depressant properties.

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Authors and Affiliations

  1. Natural Products Research Institute, Seoul National University, 110-460, Seoul, Korea

    Kuk Hyun Shin, Ok Nam Kim & Won Sick Woo

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  1. Kuk Hyun Shin
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  2. Ok Nam Kim
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  3. Won Sick Woo
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Additional information

Part 12 in the series “Studies on crude drugs acting on drug-metabolizing enzymes”. For part 11 see ref 1.

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Shin, K.H., Kim, O.N. & Woo, W.S. Isolation of hepatic drug metabolism inhibitors from the seeds ofMyristica fragrans . Arch. Pharm. Res. 11, 240–243 (1988). https://doi.org/10.1007/BF02861315

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  • DOI: https://doi.org/10.1007/BF02861315

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