Abstract
A ginseng protein fraction which has been reported to have radiation protective effect was purified from Korean ginseng and its effects on relative survival and chromosome aberration were studied in UV irradiated CHO-K1 cells. When the protein fraction (100 μg/ml) was added to the cells before UV irradiation at 4 J/m2, the survival rates were increased to 53.8% from 40.6% in control. Addition of the protein (100 μg/ml) after UV irradiation at 4 and 8 J/m2 raised the rates to 85.4 and 24.0% from 79.2 and 11.5% in control, respectively. When the ginseng protein (800 μg/ml) was added to the cells exposed to UV light at 10, 20, 30 J/m2, the frequencies of chromosome aberration (CA) were reduced significantly to almost same level regardless of the UV dose increment and there was no significant difference between pre- and post-treatment. When the concentration of ginseng protein was increased from 200 to 800 μg/ml, at UV dose of 10, 20, 30 J/m2 each, the CA frequencies were decreased consistently as the dose of ginseng protein increased, at all UV doses tested. Similar effects were observed in both cases of pre- and post-treatment. The data suggest that the protein may reduce cell damage caused by UV light, especially damage to DNA molecule, or play a role in repair processes of damaged DNA, to increase cell survival and reduce chromosome aberrations.
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Literature Cited
Takeda, A., Yonezawa, M. and Katoh, N.: Restoration of radiation injury by ginseng. I. Responses of X-irradiated mice to ginseng extract.J. Radiat. Res. 22, 323 (1981).
Takeda, A., Katoh, N. and Yonezawa, M.: Restoration of radiation injury by ginseng. III. Radioprotective effect of thermostable fraction of ginseng extract on mice, rats and guinea gigs.J. Radiat. Res. 23, 150 (1982).
Oura, H., Hiai, S., Odaka, Y. and Yokozawa, T.: Studies on the biochemical action of ginseng saponin.J. Biochem. 77, 1057 (1975).
Yonezawa, M., Takeda, A. and Katoh, N.:Proc. 3rd Inter. Ginseng Symp. pp. 17–20 (1980).
Yonezawa, M., Takeda, A. and Katoh, N.: Restoration of radiation injury by ginseng. II. Some properties of radioprotective substances.J. Radiat. Res. 22, 336 (1981).
Kim, C. and Han, G.S.: Radioprotective effects of ginseng proteins.J. Pharm. Soc. Korea 29, 246 (1985).
Kim, C. and Chun, M.Y.: Interaction between radioprotective ginseng proteins and nucleic acid.J. Korean Res. Inst. Better Liv. 38, 193 (1986).
Kim, C. and Choi, J.E.: Effects of radioprotective ginseng protein on UV induced sister chromatid exchanges.Arch. Pharm. Res. 11(2), 93 (1988).
Puck, T.T. and Marcus, P.I.,J. Exptl. Med. 103, 653 (1956).
Rauth, A.M.:Radiation Res. 31, 121 (1967).
Maher, V.M., Birch, N., Otto, R. and McCormic, J.J.:J. Natl. Cancer Inst. 54, 1287 (1975).
Haseltine, W.A.:Cell 33, 13 (1983).
Reynolds, R.J., Natarajan, A.T. and Lohman, P.H.M.: Micrococcus Leteus UV-endonuclease sensitive sites and sister chromatid exchanges in Chinese hamster ovary cells.Mut. Res. 64, 353 (1979).
Bender, M.A., Griggs, H.G. and Walker, P.L.:Mut. Res. 20, 387 (1973).
Hart, R.W., Setlow, R.B. and Woodhead, A.D.:Proc. Natl. Acad. Sci. USA. 74(12), 5574 (1977).
Rotman, R.H. and Seolow, R.B.:Photo Chemistry and Photobiology.29, 57 (1979).
Kato, H.: Photoreactivation of sister chromatid exchanges induced by ultraviolet irradiation.Nature 249, 552 (1974).
Bender, M.A., Griggs, H.G. and Walker, P.L.: Mechanism of chromosomal aberration production.Mut. Res. 20, 387 (1973).
Casarett, A.P.:Radiation Biology, Prentice Hall, pp. 152–155 (1968).
Kim, C. and Hwang, J.J.: Polyacrylamide gel electrophoresis on ginseng protein.J. Pharm. Soc. Korea 30, 343 (1986).
Pattern, M.K. Jr.: Measurement of growth and viability of cells in culture.Method in Enzymology 58, 141 (1979).
Bhayan, B.K., Loughan, B.E., Fraser, T.J. and Day, K.J.: Comparison of different methods of determining cell viability after exposure to cytotoxic compounds.Exp. Cell Res. 97, 275 (1976).
Evans, H.J.: inProgress in Genetic Toxicity Elsevier/North Holland, Amsterdam, pp. 24–51 (1977).
Kim, C. and Yoon, S.R.: Cytotoxic effect of radioprotective ginseng protein fraction on CHO-KI cells.J. Pharm. Soc. Korea,32(5), 11 (1988).
Evans, H.J. and Scott, D.: Influence of DNA synthesis on the production of chromatid aberations by X-rays and maleic hydrazide inVicia faba.Genetics 49, 17 (1964).
Scott, D.: inChromosomes Today Vol. 6, Elsevier/North Holland, Biomedical Press, pp. 391–401 (1977).
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This work was supported in part by the research grant from Korea Science and Engineering Foundation.
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Kim, C., Park, S.Y. Effects of ginseng protein on relative survival and chromosome aberration of UV irradiated cells. Arch. Pharm. Res. 11, 225–229 (1988). https://doi.org/10.1007/BF02861313
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DOI: https://doi.org/10.1007/BF02861313