Abstract
Na+−Ca2+ exchange process in sarcolemmal vesicles isolated from mesenteric arteries of Wistar-Kyoto normotensive(WKY) and spontaneously hypertensive rats(SHR) was investigated. The sarcolemmal fractions isolated after homogenization and sucrose density gradient centrifugation were enriched with 5′-nucleotidase and ouabain sensitive, K+-dependent phosphatase activities. When the vesicles were loaded with Na+, a time dependent Ca2+ uptake was observed. However, very little Ca2+ uptake was observed when the vesicles were loaded with K+, or Ca2+ uptake of the Na+-loaded vesicles was carried out in high sodium medium so that there was no sodium gradient. When the vesicles loaded with Ca2+ by Na+−Ca2+ exchange were diluted into potassium medium containing EGTA, Ca2+ was rapidly released from the vesicles. Na+-dependent Ca2+ uptake was increased in SHR compared to WKY, but passive efflux of preaccumulated Ca2+ from the vesicles was decreased in SHR. The data indicate that the membrane vesicles of rat mesenteric arteries exhibit Na+−Ca2+ exchange activity. It is also suggested that changes of this process in vascular smooth muscle cell membrane of SHR may be involved in higher intracellular Ca2+ concentration and higher basal tone in SHR.
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Lee, S.W., Lee, J.S., Park, Y.J. et al. Increase in Na+−Ca2+ exchange activity in sarcolemma isolated from mesenteric arteries of spontaneously hypertensive rats. Arch. Pharm. Res. 12, 128–134 (1989). https://doi.org/10.1007/BF02857736
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DOI: https://doi.org/10.1007/BF02857736