Skip to main content
SpringerLink
Log in

Multicomponent analysis of metabolites of low volatility in biological fluids by field ionization mass spectrometry

  • Original Articles
  • Published:
Archives of Pharmacal Research Aims and scope Submit manuscript

Abstract

An improved mass spectrometric method for multicomponent analysis of metabolities in urine, well-suited for clinical biochemistry, is described. The method involves solvent elution of the metabolites from an adsorbent and the concentration of the eluate on a microadsorption column. This is administered by a direct inlet probe into the ionizing source of field ionization mass spectrometry (FIMS), which yield a molecular weight profile of the metabolites. The procedure provides rapidly (within one hour) reproducible profiles from a small volume of urine. The optimization of the sampling technique and the reproducibility are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Literature Cited

  1. Stafford, M., Horning, M.G., and Zlatkis, A.: Profiles of volatile metabolites in body fluids.J. Chromatogr. 126, 495 (1976).

    Article  PubMed  CAS  Google Scholar 

  2. Politzer, I.R., Dowty, B.J., and Laseter, J.L.: Use of gas chromatography and mass spectrometry to analyze underivatized volatile human or animal constituents of clinical interest.Clin. Chem. 22, 1775 (1976).

    PubMed  CAS  Google Scholar 

  3. Seta, K., Washitake, M., Anmo, T., Takai, M., and Okuyama, T.: High performance anion-exchange chromatography of UV-absorbing constituents of human urine.J. Chromatogr. 181, 311 (1980).

    Article  PubMed  CAS  Google Scholar 

  4. Zlatkis, A., Brazeil, R. S., and Poole, C. F.: The role of organic volatile profiles in clinical diagnosis.Clin. Chem. 27, 789 (1981).

    PubMed  CAS  Google Scholar 

  5. Rhodes, G., Miller, M., McConnell, M.L., and Novotny, M.: Metabolic abnormalities associated with diabetes mellitus as investigated by gaschromatography and pattern-recognition analysis of profiles of volatile metabolites.Clin. Chem. 27, 580 (1981).

    PubMed  CAS  Google Scholar 

  6. Anbar, M. and Aberth, W.H.: Field ionization mass spectrometry, a new tool for the analytical chemist.Anal. Chem. 46, 59A (1974).

    Article  CAS  Google Scholar 

  7. Anbar, M., Dyer, R.L., and Scolnick, M.E.: Diagnosis of infectious hepatitis by multicomponent analysis with use of field ionization mass spectrometry.Clin. Chem. 22, 1503 (1976).

    PubMed  CAS  Google Scholar 

  8. Abbott, R., Anbar, M., Faden, M., McReynolds, J., Rieth, W., Scanlon, M., Verkh, L. and Wolff, B.: Diagnosis of viral infections by multicomponent mass spectrometric analysis.Clin. Chem. 26, 1443 (1980).

    PubMed  CAS  Google Scholar 

  9. Aberth, W., Marcuson, R., Barth, R., Emond, R., Dunham, W.B.: Profile analysis of volcano field ionization mass spectra of urine from mice with sarcoma I transplanted by intraperitoneal inoculation.Biomed Mass Spectrom. 10, 89 (1983).

    Article  PubMed  CAS  Google Scholar 

  10. Cross, R.H., Brown, H.L., and Anbar, M.: Preactivated highly efficient linear field ionization source.Rev. Sci. Instrum. 47, 1270 (1977).

    Article  Google Scholar 

  11. McReynolds, J.H., Flynn, N.W., Sperry, R.R., et al.: Glass lined field ionization sources.Anal. Chem. 49, 2121 (1977).

    Article  CAS  Google Scholar 

  12. Savitzky, A. and Golay, M.J.E.: Smoothing and differentiation of data by simplified least squares procedures.Anal. Chem. 36, 1627 (1964).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Pharmacy, Sung Kyun Kwan University, 170, Suweon, Korea

    Kyoung Rae Kim

  2. Department of Biophysical Sciences, School of Medicine, State University of New York at Buffalo, 14124, Buffalo, N. Y., USA

    Michael Anbar

Authors
  1. Kyoung Rae Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael Anbar
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kim, K.R., Anbar, M. Multicomponent analysis of metabolites of low volatility in biological fluids by field ionization mass spectrometry. Arch. Pharm. Res. 7, 23–31 (1984). https://doi.org/10.1007/BF02856918

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02856918

Keywords

Search

Navigation