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Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer

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Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1996))

Abstract

Ultra performance liquid chromatography-mass spectrometry (UPLC-MS) is the most frequently applied analytical platform in the untargeted metabolomic study of mammalian urine. Here we describe two complementary UPLC-MS methods for metabolomic analysis or urine, a reversed phase C18 method and a hydrophilic interaction liquid chromatography (HILIC) method. We discuss the inclusion of pooled quality control (QC) samples and a recommended analysis list construction. Up to 96 injections can be performed every 24 h, and up to 2000 metabolites can be routinely detected.

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Acknowledgments

This work was partly funded through a Medical Research Council funded grant in the UK to construct Phenome Centre Birmingham (MR/M009157/1).

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Authors and Affiliations

  1. School of Biosciences, University of Birmingham, Birmingham, UK

    Lukáš Najdekr & Warwick B. Dunn

  2. Phenome Centre Birmingham, University of Birmingham, Birmingham, UK

    Lukáš Najdekr & Warwick B. Dunn

  3. Beatson Institute for Cancer Research, Glasgow, UK

    Giovanny Rodriguez Blanco

  4. Institute of Metabolism and Systems Research, University of Birmingham, Birmingham, UK

    Warwick B. Dunn

Authors
  1. Lukáš Najdekr
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  2. Giovanny Rodriguez Blanco
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  3. Warwick B. Dunn
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Corresponding author

Correspondence to Lukáš Najdekr .

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Editors and Affiliations

  1. Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA

    Sanjoy K. Bhattacharya

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Najdekr, L., Blanco, G.R., Dunn, W.B. (2019). Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer. In: Bhattacharya, S. (eds) Metabolomics. Methods in Molecular Biology, vol 1996. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9488-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9488-5_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9487-8

  • Online ISBN: 978-1-4939-9488-5

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