Abstract
The indirect fluorescent antibody staining (IFAS) procedure was successfully used in place of the Gram stain procedure to confirm diagnosis of bacterial ring rot. Antisera produced against untreatedCorynebacterium sepedonicum cells, glutaraldehyde-fixed cells, heat-treated glutaraldehydefixed cells, and a cell extract had useful IFAS titers and similar specificity. Optimum antiserum dilution was critical for a reliable test; at high antiserum dilution optimum antigen excess caused poor staining, while at low antiserum dilution non-specific reactions occurred. Although the IFAS procedure was useful as a confirmatory test, its realiability as a diagnostic test is seriously questioned.
Resumen
La prueba de tinción indirecta de anticuerpo fluoroscente (TIAF) fue exitosamente utilizada en vez de la tinción de Gram para confirmar el diagnóstico de la podredumbre anular bacteriana. Antisuero producido contra células deCorynebacterium sepedonicum sin tratar, células fijadas con glutaraldehydo, células tratadas con calor y fijadas con glutaraldehydo y un extracto de células tuvieron títulos TIAF útiles y especificidad similar. Fue crítica para una prueba confiable, la dilución de antisuero; a dilución alta de antisuero el exceso de antígeno produjo tinción pobre, mientras que a dilución baja de antisuero hubieron reacciones no específicas. Aunque el procedimiento TIAF fue útil como prueba confirmativa, su confiabilidad como prueba diagnóstica es seriamente puesta en duda.
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De Boer, S.H., Copeman, R.J. Bacterial ring rot testing with the indirect fluorescent antibody staining procedure. American Potato Journal 57, 457–465 (1980). https://doi.org/10.1007/BF02854461
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DOI: https://doi.org/10.1007/BF02854461