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In vitro conservation of potato germplasm at the International Potato Center

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Abstract

The last decade has seen growing awareness of the value of germplasm conservation.In vitro maintenance of germplasm can offer many advantages as a complement to field maintenance. The International Potato Center (CIP) has actively researched a wide range of methods for effectivein vitro maintenance in its large potato germplasm collection. This review describes the methodology applied by CIP and discusses new research now being undertaken to study the genetic stability of these storage methods.

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Literature Cited

  1. Addicott, F.T. and J.L. Lyon. 1969. Physiology of abscisic acid and related substances. Ann Rev Pot Physiol 20: 139–154.

    Article  CAS  Google Scholar 

  2. Bajaj, Y.P.S. 1981. Regeneration of plants from potato meristem freeze preserved for 24 months. Euphytica 30: 141–145.

    Article  Google Scholar 

  3. Bajaj, Y.P.S. and J. Reinert. 1977. Cryobiology of plants cell cultures, and establishment of gene banks.In: Applied and fundamental aspects of plant cell tissue and organ culture (J. Reinert and YPS Bajaj, Eds.). Spring-Verlag. Berlin. pp. 757–776.

    Google Scholar 

  4. Buckner, B. and B.B. Hyde. 1985. Chloroplast DNA variation between the common cultivated potato (Solanum tuberosum spp.tuberosum) and several South American relatives. Theor Appl Genet 71: 527–531.

    Article  CAS  Google Scholar 

  5. Beckman, J.S. and M. Soller. 1984. Restriction fragment length phymorphisms in genetic improvement: Methodologies, mapping and costs. Theor Appl Genet: 67: 35–43.

    Article  Google Scholar 

  6. Bernatzky, R. and S.D. Tanksley. 1986. Toward a saturated linkage map in tomato based on isozymes and random DNA sequences. Genetics 112: 887–878.

    PubMed  CAS  Google Scholar 

  7. Desborough, S. and S.J. Peloquin. 1969. Tuber proteins from haploids, selfs, and cultivars of group tuberosum separated by acid gel disc electrophoresis. Theor Appl Genet 39: 43–47.

    Article  Google Scholar 

  8. Dodds, J.H. 1986. Storage of Plant Genetic Resources.In: Experiments in Plant Tissue Culture (J.H. Dodds and L.W. Roberts, Eds.). Cambridge Press. pp. 172–180.

  9. Estrada, R., P. Tovar and J.H. Dodds. 1986. Induction ofin vitro tubers in a broad range of potato genotypes. Plant Cenn Tissue and Organ Culture 7: 3–10.

    Article  Google Scholar 

  10. Frankel, O.H. and J.G. Hawkes. 1975. Crop Genetic Resources for Today and Tomorrow. Cambridge Press.

  11. Glover, D. M. 1980. Genetic Engineering Cloning DNA. Chapman and Hall. pp. 48–66.

  12. Grout, B.W.W. and G.G. Henshaw. 1978. Freeze preservation of potato shoot tip cultures. Ann Bot 42: 1227–1229.

    Google Scholar 

  13. Harmey, M.A., M.P. Crawley and P.E.M. Clinch. 1966. The effect of growth regulators on the tuberisation of cultured stem pieces ofS. tuberosum. Eur Potato J 9: 146–151.

    Article  CAS  Google Scholar 

  14. Henshaw, G.G. 1979. Tissue cultures and germplasm storage. IAPTC newsletter 28: 2–7.

    Google Scholar 

  15. Henshaw, G.G. and J.F. O’Hara. 1983.In vitro approaches to the conservation and utilisation of global plant genetic resources.In: Plant Biotechnology (S.H. Mantell and H. Smith, Eds.). C.U.P. pp. 219–238.

  16. Humphries, E.C. and E.C. Dyson. 1967. Effect of growth inhibitor N-Dimethylamino Succinamic acid (B9) on potato plants in the field. Eur Potato J 10: 116–126.

    Article  CAS  Google Scholar 

  17. Kanai, R. and G.E. Edwards. 1973. Purification of enzymically isolated protoplasts from C3m C4 and CAM plants using an aqueaous dextran-polyethylene glycol two phase system. Plant Physiol 52: 484–490.

    Article  PubMed  CAS  Google Scholar 

  18. Kartha, K.K. 1985. Meristem culture and Germplasm Preservation.In: Cryopreservation of Plant Cells and Organs (CRC Press, Ed.). pp. 116–134.

  19. Kartha, K.K., N.L. Leung and O.L. Gamborg. 1979. Freeze preservation of pea meristems in liquid nitrogen and subsequent plant regeneration. Plant Sci Lett 15: 7–15.

    Article  Google Scholar 

  20. Larkin, P.J. and W.R. Scowcroft. 1981. Somaclonal variation—a novel source of genetic variability from cell cultures for improvement. Theor App Gen 60: 197–214.

    Article  Google Scholar 

  21. Maliga, P. 1978. Resistance mutants and their use in genetic manipulation.In: Frontiers of Plant Tissue Culture (T.A. Thorpe, Ed.). IAPTC. pp. 381–392.

  22. Morel, G. 1975. Meristem culture techniques for long term storage of cultivated plants.In: Crop Genetic Resources for Today and Tomorrow (O.H. Frankel and J.G. Hawkes, Eds.). C.U.P. pp. 327–332.

  23. Nitzsche, W. 1983. Germplasm Preservation.In: Handbook of Plant Cell Culture (D.A. Evans, W.R. Sharp, P.V. Ammirato and Y. Yamada, Eds.). Macmillan, New York, pp. 782–805.

    Google Scholar 

  24. Paupardin, C. and R. Tizio. 1970. Sur la présence de composés phenoliques dans les germes de pomme de terra cultivesin vitro. C.R. Acad Sci Ser D Natur (Paris) 269: 1668–1670.

    Google Scholar 

  25. Roca, W.M., N.O. Espinoza, M.R. Roca and E.J. Bryan. 1978. A tissue culture method for rapid propagation of potatoes. Am Potato J 55: 691–701.

    Google Scholar 

  26. Sadatz, W. and K.H.C. Standke. 1978. Untersuchungan Minimalwachstum von Kartoffelnin vitro. Landbauforschung Volksnnode 28: 75–78.

    Google Scholar 

  27. Scowcroft, W.R. and P.J. Larking. 1982. Somaclonal Variation: A new option for plant improvement.In: Plant Improvement and Somatic Gel Genetics (J.K. Vasil, W.R. Scowcroft and K.J. Frey, Eds.). Academic Press pp. 159–178.

  28. Silva, G.H. 1985.In vitro storage of potato tuber expiants and subsequent plant regeneration. HortScience 20(1): 139–140.

    Google Scholar 

  29. Stecco, V.L. et R. Tizio. 1982. Cetion du CCC sur la tuberisation des germes de Pomme de terre cultivésin vitro caun milieu minéral dé pour vu de sucre. C R Acad Sc Serie III 294: 901–904.

    Google Scholar 

  30. Stegemann, H. 1979. Characterisation of Proteins from Potatoes and the “Index of European Varieties.” Conference The Biology and Taxonomy of the Solanacea, July 1976. (J.G. Hawkes, R.N. Lester and A.D. Skelding, Eds.). Linnean Society Symposium Series. 7:279–284.

  31. Stegemann, H., H. Francksen and V. Macko. 1973. Potato proteins: genetic and physiological changes, evaluated by one- and two-dimensional PAA-gel-techniques. Z. Naturforach 28c(11-12): 722–732.

    Google Scholar 

  32. Thomas, E. 1981. Plant regeneration from shoot culture derived protoplasts to tetraploid potato. Plant Sci Lett 23: 81–88.

    Article  Google Scholar 

  33. Westcott, R.J., G.G. Henshaw and B.W.W. Grout. 1977. Tissue Culture Methods and Germplasm Storage in Potato. Acta Horticulturae 78: 45–49.

    Google Scholar 

  34. Westcott, R.J., G.G. Henshaw and W.M. Roca. 1977. Tissue culture storage of potato germplasm: Culture initiation and plant regeneration. Plant Sci Lett 9: 309–315.

    Article  CAS  Google Scholar 

  35. Wilkins, C.P. and J.H. Dodds. 1983. The application of tissue culture techniques to plant genetic conservation. Sci Prog 68: 281–307.

    Google Scholar 

  36. Withers, L.A. 1983. Germplasm storage in plant biotechnology.In: Plant Biotechnology (S.H. Mantell and H. Smith, Eds.). Cambridge Press. pp. 187–218.

  37. Withers, L.A. 1980. Tissue Culture Storage for Genetic Conservation. IBPGR technical report. AGP/80/8 Rome. 91 pp.

  38. Withers, L.A. and H.E. Street. 1977. Freeze preservation of plant cell cultures.In: Plant Tissue Culture and its Biotechnological Applications (W. Barz, E. Reinert and M.H. Zenk, Eds.). Springer-Velag. pp. 226–244.

  39. Withers, L.A. and M.R. Davey. 1978. A fine structural study of the freeze preservation of plant tissue cultures. Protoplasma 94: 207–219.

    Article  Google Scholar 

  40. Withers, L.A. and P.J. King. 1979. Proline—A novel cryoprotectant for the freeze preservation of cultured cells ofZea mays. Plant Physiol 64: 675–678.

    PubMed  CAS  Google Scholar 

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Lizarraga, R., Huaman, Z. & Dodds, J.H. In vitro conservation of potato germplasm at the International Potato Center. American Potato Journal 66, 253–269 (1989). https://doi.org/10.1007/BF02853449

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