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Diagnosis of tobacco rattle virus (TRV) and other viruses in weed and rotation crops in potato fields

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Abstract

The methodology for the detection of tobacco rattle virus (TRV) and for the incidence of TRV and other viruses was determined in weed species associated with potatoes.

Symptoms onNicotiana tabacum L. cv. ‘Samsun NN’and Phaseolus vulgaris L. cv. ‘Bountiful’ were used to detect the viruses. Identity was confirmed by comparison with symptoms produced on plants by standard cultures of TRV and potato virus X (PVX), by electron microscopy, and by serology.Portulaca oleracea L.,Solatium sarachoides Sendt., andAmaranthus retroflexus L. were hosts of TRV; the latter two also carried PVX. Most TRV carriers were found adjacent to infected potatoes. The roots of naturally infectedS. sarachoides contained the highest concentration of TRV encountered in these studies. Mechanical inoculation of TRV to A.retroflexus andS. sarachoides produced variable symptoms that included red spots on the former and chlorosis and stunting on the latter. TRV moved systematically in both species. Assay methods for detection were adequate but low concentrations of TRV limited the usefulness of electron microscopy and serology.

Resumen

La metodología para la detección del virus rattle del tabaco (TRV) y la incidencia de TRV y otros virus fue determinada en malezas asociadas con papa.

Síntomas enNicotiana tabacum L. cv. ‘Samsun NN’ yPhaseolus vulgaris L. cv. ‘Bountiful’ fueron usados para detectar los virus. La identidad fue confirmada comparando los síntomas producidos en plantas por aislamientos tipicos de TRV; y al virus X de la papa (PVX) por microscopia electrónica y serología.Portulaca oleracea L.,Solatium sarachoides Sendt., yAmaranthus retroflexus L. fueron huéspedes de TRV; los dos últimos también lo fueron de PVX. La mayoría de plantas infestadas con TRV fueron halladas adyacentes a papas infectadas. Las raíces deS. sarachoides naturalmente infectadas tuvieron la más alta concentración de TRV entre las halladas en estos estudios. Inoculación mecánica de TRV aA.retroflexus yS. sarachoides produjo síntomas variables que incluían manchas rojas en el primero y clorosis y detención del crecimiento en el segundo. TRV se hizo sistémico en ambas especies. El método de infectividad para la detención de TRV fue adecuado pero la baja concentratión de TRV limitó la utilidad de microscopía electrónica y serología.

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Authors and Affiliations

  1. Oregon State University, USA

    E. A. Locatelli, T. C. Allen (Plant Pathologist), P. A. Koepsell (Associate Extension Plant Pathologist) & A. P. Appleby (Agronomic Crop Scientist)

  2. Centro Agronomic Tropical de Investigacion y Ensenanza, Turrialba, Costa Rica

    E. A. Locatelli

Authors
  1. E. A. Locatelli
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  2. T. C. Allen
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  3. P. A. Koepsell
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  4. A. P. Appleby
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Additional information

Technical Paper No. 4632. Agricultural Experiment Station, Corvallis, OR 97331.

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Locatelli, E.A., Allen, T.C., Koepsell, P.A. et al. Diagnosis of tobacco rattle virus (TRV) and other viruses in weed and rotation crops in potato fields. American Potato Journal 55, 249–257 (1978). https://doi.org/10.1007/BF02852133

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  • DOI: https://doi.org/10.1007/BF02852133

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