Abstract
Investigations on the ecological function of ineffectiveFrankia strains and their behaviour in competition with effectiveFrankia strains indicated an enhanced plant growth upon dual inoculation with increasing amounts of effective (i.e. N2-fixing)Frankia strains and simultaneous inoculation with a constant amount of an ineffectiveFrankia strain. Enhanced plant growth was measured as increase in plant height and total dry weight at constant shoot/root ratio. The stimulating effect of the ineffective strain was independent of the plant clone and was obtained with bothAlnus glutinosa clones W I and B II, which were resistant and susceptable, respectively, to the ineffective strain. Stimulation was also independent of the nodulation conditions. Short-term studies (7 weeks) under axenic conditions and greenhouse experiments during 3 months showed comparable results, not only in plant growth but also in nodule formation. Increment in plant growth was not necessarily correlated to higher nodule formation with the effectiveFrankia strains.
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Bermudez de Castro F, Canizo A, Costa A, Miguel C and Rodriguez-Barrueco C 1977 Cytokinins and nodulation of the non-legumesAlnus glutinosa andMyrica gale.In Recent Developments in Nitrogen Fixation. Eds. W Newton, J R Postgate and C Rodriguez-Barrueco. pp 539–550. Academic Press, London.
Capellano A, Dequatre B, Valla G and Moiroud A 1987 Root-nodules formation byPenicillium sp. onAlnus glutinosa andAlus incana. Plant and Soil 104, 45–51.
Dijk C van 1984 Ecological Aspects of Spore Formation in theFrankia-Alnus Symbiosis. PhD thesis, Univ. Leiden, The Netherlands.
Dijk C van and Sluimer-Stolk A 1984 An ineffective strain ofFrankia-Ag in the dune area of Voorne. Progr. Rep. Inst. Ecol. Res. Verh. Kon. Ned. Akad. Wetensch., 2e Reeks 82, 56–59.
Dijk C van and Sluimer-Stolk A An ineffective strain type ofFrankia in the soil of natural sands ofAlnus glutinosa (L.) Gaertn. Plant and Soil. (Submitted).
Dobritsa S V and Sharaya L S 1986 Genome identity of differentNocardia autotrophica isolates fromAlnus spp. root nodules and rhizosphere.In Biological, Biochemical and Biomedical Aspects of Actinomycetes. Eds. G Szabo, S Biro and M Goodfellow. pp 497–506. Akademiai Kiado, Budapest.
Gardner I C, Clelland D M and Scott A 1984 Mycorrhizal improvement in non-leguminous nitrogen fixing associations with particular reference toHippophaë rhamnoides L. Plant and Soil 78, 189–199.
Garton S, Hosier M A, Read P E and Farnham R S 1981In vitro propagation ofAlnus glutinosa Gaertn. Hort Science 16, 758–759.
Griffiths A P and McCormick L H 1984 Effects of soil acidity on nodulation ofAlnus glutinosa and viability ofFrankia. Plant and Soil 79, 429–434.
Hahn D, Starrenburg M J C and Akkermans A D L 1988 Variable compatibility of clonedAlnus glutinosa ecotypes against ineffectiveFrankia strains. Plant and Soil 107, 233–243.
Hahn D, Dorsch M, Stackebrandt E and Akkermans A D L 1989 Use of synthetic oligonucleotide probes for identification ofFrankia strains. Plant and Soil 118, 211–219.
Heller R 1953 Recherches sur la nutrition minérale des tissues végétaux cultivésin vitro. Thèse, Paris, 223 p.
Knowlton S, Berry A and Torrey J C 1980 Evidence that associated soil bacteria may influence roothair infection of actinorhizal plants byFrankia. Can. J. Microbiol. 26, 971–977.
Knowlton S and Dawson J O 1983 Effects ofPseudomonas cepacia and cultural factors on the nodulation ofAlnus rubra roots byFrankia. Can. J. Bot. 61, 2877–2882.
Lechevalier M P, Baker D and Horrière F 1983 Physiology, chemistry, serology, and infectivity of twoFrankia isolates fromAlnus incana subsp.rugosa. Can. J. Bot. 61, 2826–2833.
MacConnell J T and Bond G 1957 A comparison of the effect of combined nitrogen on nodulation in non-legumes and legumes. Plant and Soil 8, 378–388.
Meesters T M, Genesen S Th van and Akkermans A D L 1985 Growth, acetylene reduction activity and localization of nitrogenase in relation to vesicle formation inFrankia strains Ccl. 17 and Cp1.2. Arch Microbiol 143, 137–142.
Moss R D and Bard R C 1957 Physiological and biochemical technics.In The Society of American Bacteriologists (Eds.) Manual of Microbiology, Methods. McGraw-Hill, New York.
Perinet P and Lalonde M 1983In vitro propagation and nodulation of the actinorhizal host plantAlnus glutinosa Gaertn. Plant Science Lett. 29, 9–17.
Prat D 1989 Effects of some pure and mixedFrankia strains on seedling growth in differentAlnus species. Plant and Soil 113, 31–38.
Simon L, Bousquet J, Gardes M, St-Laurent L and Lalonde M 1988 Competitiveness ofFrankia strains onElaeagnus clonal plantlets. FEMS Microbiol. Lett. 51, 13–18.
Smolander A and Sundman V 1987Frankia in acid soils devoid of actinorhizal plants. Physiol. Plant. 70, 297–303.
Smolander A, Dijk C van and Sundman V 1988 Survival ofFrankia strains introduced into soil. Plant and Soil 106, 65–72.
Stewart W D P and Bond G 1961 The effect of ammonium nitrogen on fixation of elemental nitrogen inAlnus andMyrica. Plant and Soil 14, 347–359.
Strzelczyk E and Pokojska-Burdziej A 1984 Production of auxins and gibberelin-like substances by mycorrhizal fungi, bacteria and actinomycetes isolated from soil and the mycorrhizosphere of pine (Pinus sylvestris L.). Plant and Soil 81, 185–194.
Strzelczyk E and Leniarska U 1985 Production of B-group vitamins by mycorrhizal fungi and actinomycetes isolated from the root zone of pine (Pinus sylvestris L.). Plant and Soil 86, 387–394.
Suetin S O, Sharaya L S, Pariiskaya A N and Kalakutskii L V 1988 Penetration ofStreptomyces globisporus into root cells ofAlnus glutinosa (L.) Gaertn. Mikrobiologiya 57, 132–136.
Tremblay F M and Lalonde M 1984 Requirements forin vitro propagation of seven nitrogen-fixingAlnus species. Plant Cell Tissue Organ Culture 3, 189–199.
Valla G, Capellano A, Hugueney R and Moiroud A 1989Penicillium nodositatum Valla, a new species inducing myconodules onAlnus roots. Plant and Soil 114, 142–146.
Verweij J A 1983 Een vergelijkend onderzoek naar de kieming en vestiging van twee ecotypen van zwarte els (Alnus glutinosa (L.) Gaertn.). Rapport Rijksinstituut voor onderzoek in de bos- en landschapsbouw ‘De Dorschkamp’, Wageningen, nr. 354.
Wheeler C T, Crozier A and Sandberg G 1984 The biosynthesis of indole-3-acetic acid byFrankia. Plant and Soil 78, 99–104.
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Hahn, D., Starrenburg, M.J.C. & Akkermans, A.D.L. Growth increment ofAlnus glutinosa upon dual inoculation with effective and ineffectiveFrankia strains. Plant Soil 122, 121–127 (1990). https://doi.org/10.1007/BF02851919
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DOI: https://doi.org/10.1007/BF02851919