Skip to main content
SpringerLink
Log in

Frequency of loss expression of DPC4 protein in various locations of biliary tract carcinoma

  • Original Articles
  • Published:
The Chinese-German Journal of Clinical Oncology

Abstract

Objective

To clarify the relationship between loss of expression of DPC4 proteins and pathogenesis of biliary tract carcinoma.

Methods

71 primary biliary tract carcinomas (BTCa), including 38 common bile duct (CBD) carcinomas, 18 gallbladder carcinomas, and 15 hilar bile ducts (HBD) carcinomas were examined by immunohistochemical staining. In addition, the CBD carcinomas were divided into two groups, a tumor group with metastasis (M+ group, 27 cases) and a tumor group without metastasis (M− group, 11 cases).

Results

The frequency of loss expression of DPC4 protein was 32.8% in BTCa, 47.3% in CBD carcinoma, 11% in gallbladder carcinoma and 13% in HBD carcinoma. A comparison of the frequency of loss expression of DPC4 showed significantly statistical difference in the CBD carcinoma versus gallbladder carcinoma and HBD carcinoma (P<0.01). The frequency of loss expression of DPC4 was 48.1% in the M+ group and 45.4% in the M− group. There was no significantly statistical difference between them (P>0.05).

Conclusion

There is a close relationship between the pathogenesis of BTCa and inactivation of DPC4 with different frequencies of DPC4 gene alteration in various locations of the biliary tract, but inactivation of DPC4 is not related with tumor metastasis in BTCa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ohashi K, Tsutsumi M, Nakajima Y, et al. K-ras point mutation and proliferation activity in biliary tract carcinoma. Br J Cancer, 1996, 74 (7): 930–935.

    PubMed  CAS  Google Scholar 

  2. Washington K, Gottfried MR. Expression of p53 in adenocarcinoma of the gallbladder and bile duct. Liver, 1996, 16(2): 99–104.

    PubMed  CAS  Google Scholar 

  3. Yoshida S, Todoroki T, Ichikawa Y, et al. Mutation of p16INK4/CDKN2 and p15INK4B/MTS2 gene in biliary tract cancers. Cancer Res, 1995, 55 (13): 2756–2760.

    PubMed  CAS  Google Scholar 

  4. Hahn SA, Bartsch D, Schroers A, et al. Mutations of the DPC4/Smad4 gene in biliary tract carcinoma. Cancer Res, 1998, 15;58(6): 1124–1126.

    Google Scholar 

  5. Wilentz RE, Su GH, Dai JL, et al. Immunohistochemistry labeling for DPC4 mirrors genetic status in pancreatic and peripancreatic adenocarcinoma: a new marker of DPC4 inactivation. Am J Pathol, 2000, 156(1): 37–43.

    PubMed  CAS  Google Scholar 

  6. Ishak KG, Anthony PP, Sobin LH. Histological typing of tumors of the gallbladder and extrahepatic bile ducts. 2nd ed, Berlin: Springer-Verlag, 1991, 102–105.

    Google Scholar 

  7. Shiraishi K, Okita K, Harada T, et al. Comparative genomic hybridization analysis of genetic aberrations associated with development and progression of biliary tract carcinomas. Cancer, 2001, 91(3): 570–577.

    Article  PubMed  CAS  Google Scholar 

  8. Hahn SA, Schutte M, Hoque AT, et al. DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1. Science, 1996, 271(5247): 350–353.

    Article  PubMed  CAS  Google Scholar 

  9. Rozenblum E, Schutte M, Goggins M, et al. Tumor suppressive pathway in pancreatic carcinoma. Cancer Res, 1997, 57(9): 1731–1734.

    PubMed  CAS  Google Scholar 

  10. Robert BA, William CC, Sreenath R. Embryology, anatomy, and surgical application of the extrahepatic biliary system. Surg Clin Nort Am, 2000, 80 (3): 363–379.

    Google Scholar 

  11. Skandalakis JE, Gray SW, Ricketts R, et al. The extrahepatic biliary ducts and the gallbladller. In Skandalakis JE, Gray SW (eds): Embryology for Surgeons. ed 2. Baltimore, Williams & Wilkins, 1994. 296–333.

    Google Scholar 

  12. Rijken AM, Hu J, Perlman EJ, et al. Genomic alterations in distal bile duct carcinoma by comparative genomic hybridization and karyotype analysis. Genes Chromosomes Cancer, 1999, 26(3): 185–191.

    Article  PubMed  CAS  Google Scholar 

  13. Miyaki M, Iijima T, Konishi M, et al. Higher frequency of Smad4 gene mutation in human colorectal cancer with distant metastasis. Oncogene. 1999, 18(20): 3098–3103.

    Article  PubMed  CAS  Google Scholar 

  14. Schwarte-Waldhoff I, Klein S, Blass-Kampmann S, et al. DPC4/SMAD4 mediated tumor suppression of colon carcinoma cells is associated with reduced urokinase expression. Oncogene. 1999, 18(20): 3152–3158.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of General Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China

    Tang Zhaohui, Zou Shengquan & Qiu Fazu

  2. Institute of Clinical Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China

    Hao Youhua & Wang Baoju

  3. Department of Biochemistry, Rheinisch-Westfalische Technische Hochschule (RWTH), D-52074, Aachen, Germany

    Yang Xiangping

Authors
  1. Tang Zhaohui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zou Shengquan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hao Youhua
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wang Baoju
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yang Xiangping
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qiu Fazu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tang Zhaohui.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tang, Z., Zou, S., Hao, Y. et al. Frequency of loss expression of DPC4 protein in various locations of biliary tract carcinoma. Chin. -Ger. J. Clin. Oncol. 1, 88–91 (2002). https://doi.org/10.1007/BF02851741

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02851741

Key words

Search

Navigation