Abstract
The cloned P1 sequence of PVYO was transferred in sense orientation into the potato cultivar Pito usingAgrobacteriummediated transformation. Sixteen of the putatively transformed plants (NPTII positive) were assayed for PVYO resistance. No PVYO was detected in four plants, representing two lines, 21 days after two sap-inoculations and 35 days after graft-inoculation, and the plants remained symptomless, whereas other tested plants showed mosaic symptoms and had high PVY titers similar to those of the control plants. No line was resistant to PVYN and potato viruses A and X. Southern analysis confirmed the presence of the transgene(s) in the two PVYO-resistant and one susceptible line examined, but no signal was detected in nontransformed Pito. These results suggest a high level of protection against PVYO in potato transformed with P1 sequence of PVYO.
Compendio
La cadena clonada del PI de PVYO fue transferida, en orientatión positiva, al cultivar Pito de papa, utilizando la transformatión mediante Agrobacterium. Dieciséis de las plantas transformadas putativamente (NPTII positivo) fueron evaluadas para resistencia a PVYO. No se detectó PVYO en cuatro plantas, representando dos líneas, 21 días después de dos inoculaciones con savia y 35 días después de inoculatión por injerto, y las plantas permanecieron sin síntomas, mientras que otras de las plantas probados mostraron síntomas de mosaico y tuvieron titulaciones altas de PVYsimilares a aquellas de las plantas testigo. Ninguna línea fue resistente al PVYN o a los virus A y X de la papa. El análisis del RNA en placas de nitrocelulosa confirmó la presencia del (o de los) transgene (s) en las dos líneas resistentes y en una susceptible al PVYO examinadas, pero no se detectó señal alguna en las plantas de Pito no transformadas. Estos resultados sugieren un alto nivel de protección contra el PVYO en la papa transformada con el encadenamiento del PI de PVYO.
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Pehu, T.M., Mäki-Valkama, T.K., Valkonen, J.P.T. et al. Potato plants transformed with a potato virus Y P1 gene sequence are resistant to PVYO . American Potato Journal 72, 523–532 (1995). https://doi.org/10.1007/BF02849255
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DOI: https://doi.org/10.1007/BF02849255