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Adoptive transfer of experimental allergic encephalomyelitis

Inhibition by agents that elevate intracellular cyclic AMP levels

  • Published:
Neurochemical Pathology

An Erratum to this article was published on 01 June 1983

Abstract

We have previously shown that adoptive transfer of experimental allergic encephalomyelitis can be greatly enhanced by culturing sensitized lymph node cells (LNC) with specific antigen, myelin basic protein (BP). In the present study, successful transfer was accomplished with 5×106 Lewis rat LNC after 48 h culture with BP. Disease transfer was inhibited by the addition to culture of dibutyryl adenosine 3’,5’-cyclic monophosphate (Bt2 cAMP) or the phosphodiesterase inhibitor, isobutyl methylxanthine (MIX), but not by Bt2cGMP. BP-induced lymphoproliferation was also inhibited, but with a slightly different dose-response relationship. An early step in the lymphocyte activation process appears to be most sensitive to the inhibitory effects of Bt2cAMP.

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Abbreviations

BP:

myelin basic protein

Bt2cAMP:

dibutyryl cyclic AMP

LNC:

lymph node cells

EAE:

experimental allergic encephalomyelitis

MIX:

isobutyl methylxanthine

CFA:

complete Freund’s adjuvant

H-T dR:

tritiated thymidine

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Author notes

  1. J. R. Richert

    Present address: Department of Neurology, Georgetown University Medical Center, Washington, D.C.

Authors and Affiliations

  1. Section on Myelin Chemistry, Laboratory of Cerebral Metabolism, National Institute of Mental Health, 20205, Bethesda, MD

    J. R. Richert & Marian W. Kies

  2. Neuropathology RJ-05, University of Washington School of Medicine, 98195, Seattle, WA

    E. C. Alvord

Authors
  1. J. R. Richert
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  2. Marian W. Kies
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  3. E. C. Alvord
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Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/BF02834197.

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Richert, J.R., Kies, M.W. & Alvord, E.C. Adoptive transfer of experimental allergic encephalomyelitis. Neurochemical Pathology 1, 81–90 (1983). https://doi.org/10.1007/BF02834133

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  • DOI: https://doi.org/10.1007/BF02834133

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