Summary
The gene and the amino acid sequence of the structural and regulatory region of thePseudomonas aeruginosa with different resistance patterns were analyzed. Six strains with different resistance patterns were selected and the AmpC β-lactamase was identified. The objective gene fragment was amplified by colonies PCR. The sequences of the PCR-products were analyzed. The DNA sequence of the structural gene ampC and the regulatory genes ampR, ampD and ampE was detected. The 6 strains and the wild-typePseudomonas aeruginosa are highly homogeneous in structural and regulatory region. Some new mutant points were found.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Sanders C C, Sanders W E. Microbial resistance to newer generatrion β-lactam antibiotics: Clinical and laboratory implications. J Infect Dis, 1985, 151(3): 399
Coudron P E, Moland E S, Thomoson K S. Occurrence and detection of AmpC beta-lactamases among Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates at a veteran's medical center. J Clin Microbiol, 2000, 38(5): 1791
Campbell J, Ciofu O, Hoiby Net al. Pseudononas aeruginosa isolates patients with cystic fibrosis have different β—lactamase expression phenotypes but are homogeneous in the ampC—ampR genetic region. Antimicrob Agents Chemother, 1997, 41(6): 1380
Taimour Y L, Gagnon L, Huletsky Aet al. Inactivation of the ampD gene inPseudonomas aeruginosa leads to moderate-basal-level and hyperinducible AmpC β-lactamase expression. Antimicrob Agents Chemother, 2000, 44(3): 583
Taimour Y L, Dargis M, Huletsky Aet al. An ampD gene inPseudononas aeruginosa encodes a negative regulator of AmpC β-lactamase expression. Antimicrob Agents Chemother, 1998, 42(12): 3296
Julia M L, Stephen D M, Piddock L Jet al. Cloning, sequencing and analysis of the structural and regulatory region of thePseudonomas aeruginosa chromosomal AmpC β-lactamase. Biochem J, 1990, 272(3): 627
Jaurin B, Grunstrom T, Normark Set al. Sequence elements determine ampC promoter strength inE. coli. EMBO J, 1982, 1(7): 875
Stapleton P, Shannon K, Phillips Iet al. DNA sequence differences of ampD mutants of Citrobacter freundii. Antimicrob Agent Chemother, 1995, 39(11): 2494
Kopp U, Wiedemann B, Lindquist Set al. Sequence of wild type and mutant ampD genes of Citrobacter freundii and Enterobacter cloae. Antimicrob Agent Chemother, 1993, 37(2): 224
Ehrhardt A F, Sanders C C, Romero J Ret al. Sequencing and analysis of four new Enterobacter ampD alleles. Antimicrob Agent Chemother, 1996, 40(8): 1953
Author information
Authors and Affiliations
Additional information
NI Ming, female, born in 1976, Doctor in Charge
This project was supported by a grant from the National Natural Sciences Foundation of China (No. 39870873).
Rights and permissions
About this article
Cite this article
Ming, N., Dongshen, Z. & Junying, Q. Analysis of AmpC β-lactamase gene inPseudomonas aeruginosa. Current Medical Science 25, 17–19 (2005). https://doi.org/10.1007/BF02831375
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02831375