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Effect of calmodulin and voltage-dependent Ca2+ channel on the proliferation of heptoma cells induced by epidermal growth factor

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Summary

The effect of thyrosine kinase, calmodulin and voltage-dependent Ca2+ channel on the proliferation of hepatoma cells induced by EGF was studied. Hepatoma cell line SMMC7721 was cultured in RPMI1640 serum-free medium. DNA synthesis rate of hepatoma cells was measured by H-TdR incorporation. 10−9 mol/L EGF could significantly stimulate the proliferation of hepatoma cells (P<0.05), and this effect might be significantly inhibited by tyrosine kinase inhibitor (P<0.001). Calmodulin inhibitor W-7 had no effect on the basic phase of cultured hepatoma cells (P>0.05), but it had very significantly inhibitory effect on the proliferation of hepatoma cells induced by EGF (P<0.001). Voltage-dependent Ca2+ channel inhibitor Varapamil had no inhibition on the proliferation of hepatoma cells induced by EGF (P>0.05). It had no effect on the basic phase of cultured hepatoma cells, (P>0.05). It is suggested that tyrosine kinase and Ca2+-calmodulin-dependent pathway may play a critical role on the proliferation of heptoma cells induced by EGF and voltage-dependent Ca2+ channel is independent of the effect of EGF.

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References

  1. Francavilla A, Barone M, Azzarone Aet al. Cell regeneration in the pathobiology of liver carcinomas. Gas-troenterology, 1991, 23: 589

    CAS  Google Scholar 

  2. Hung W C, Chuang L Y, Tsai J Het al. Effects of epidermal growth factor on growth control and signal transduction pathways in different human hepatoma cell lines. Biochem Mol Biol Int, 1993, 30(2): 319

    CAS  PubMed  Google Scholar 

  3. Gilligan A, Prentki M, Glennon M Cet al. Epidermal growth factor-induced increase in inositol trisphosphates, inositol tetrakisphosphates, and cytosolic Ca2+ in a human hepatocellular carcinoma-derived cell line. FEBS Lett, 1988, 233: 41

    Article  CAS  PubMed  Google Scholar 

  4. Nakajima M, Murata T, Yamaguchi M. Expression of calcium-binding protein regucalcin mRNA in the cloned rat hepatoma cell (H4-I-E) is stimulated through Ca2+ signal factor: involvement of protein kinase C. Mol Cell Biochem, 1999, 198(1–2): 101

    Article  CAS  PubMed  Google Scholar 

  5. Tamura N, Tai Y, Sugimoto Ket al. Enhanced expression and activation of Ca2+/calmodulin-dependent protein kinase N in hepatocellular carcinoma. Cancer, 2000, 89 (9): 1910

    Article  CAS  PubMed  Google Scholar 

  6. Grandis J R, Drenning S D, Chakraborty Aet al. Requirement of Stat 3 but not Stat1 activation for epidermal growth factor receptor-mediated cell growth in Vitro. J Clin Invest, 1998, 102(7): 1385.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Institute of Liver Disease, Tongji Hospital, Huazhong University of Science and Technology, 430030, Wuhan

    Wu Binwen, Wang Jialong & Yuan Shunyu

  2. Department of Nuclear Medicine, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan

    Cui Wuren

Authors
  1. Wu Binwen
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  2. Wang Jialong
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  3. Yuan Shunyu
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  4. Cui Wuren
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Additional information

WU Binwen, male, born in 1968, M. D., Ph.D.

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Binwen, W., Jialong, W., Shunyu, Y. et al. Effect of calmodulin and voltage-dependent Ca2+ channel on the proliferation of heptoma cells induced by epidermal growth factor. Current Medical Science 23, 26–28 (2003). https://doi.org/10.1007/BF02829454

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  • DOI: https://doi.org/10.1007/BF02829454

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