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Thermostable glucose-tolerant glucoamylase produced by the thermophilic fungusScytalidium thermophilum

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Abstract

Glucoamylase produced byScytalidium thermophilum was purified 80-fold by DEAE-cellulose, ultrafiltration and CM-cellulose chromatography. The enzyme is a glycoprotein containing 9.8% saccharide, pI of 8.3 and molar mass of 75 kDa (SDS-PAGE) or 60 kDa (Sepharose 6B). Optima of pH and temperature with starch or maltose as substrates were 5.5/70 °C and 5.5/65 °C, respectively. The enzyme was stable for 1 h at 55 °C and for about 8 d at 4 °C, either at pH 7.0 or pH 5.5. Starch, amylopectin, glycogen, amylose and maltose were the substrates preferentially hydrolyzed. The activity was activated by 1 mmol/L Mg2+ (27%), Zn2+ (21%), Ba2+ (8%) and Mn2+ (5%).K m and {ie11-1} values for starch and maltose were 0.21 g/L, 62 U/mg protein and 3.9 g/L, 9.0 U/mg protein, respectively. Glucoamylase activity was only slightly inhibited by glucose up to a 1 mol/L concentration.

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Authors and Affiliations

  1. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, 14.040-901, Ribeirão Preto, São Paulo, Brasil

    A. C. M. M. Aquino, J. A. Jorge, H. F. Terenzi & M. L. T. M. Polizeli

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  1. A. C. M. M. Aquino
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  2. J. A. Jorge
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  3. H. F. Terenzi
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  4. M. L. T. M. Polizeli
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Correspondence to M. L. T. M. Polizeli.

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Aquino, A.C.M.M., Jorge, J.A., Terenzi, H.F. et al. Thermostable glucose-tolerant glucoamylase produced by the thermophilic fungusScytalidium thermophilum . Folia Microbiol 46, 11–16 (2001). https://doi.org/10.1007/BF02825876

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  • DOI: https://doi.org/10.1007/BF02825876

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