Summary
A bioassay is described which is dependent upon the fact that gibberellin induced α-amylase release from barley half-seeds is proportional to the logarithm of gibberellin concentration applied. This bioassay has been successfully applied to the estimation of gibberellin-like substances in plant extracts. The bioassay has the following advantages: 1) Release of α-amylase is one step closer to the primary site of action of GA; 2) release of α-amylase is not affected by solvent residues and is apparently completely specific for gibberellin; 3) release of α-amylase is not affected by substances other than gibberellins present in crude plant extracts.
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Supported by U.S. Atomic Energy Commission Contract No. AT (11-1)-1338.
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Jones, R.L., Varner, J.E. The bioassay of gibberellins. Planta 72, 155–161 (1966). https://doi.org/10.1007/BF00387479
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DOI: https://doi.org/10.1007/BF00387479