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Cereal DNA: A rapid high-throughput extraction method for marker assisted selection

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Abstract

A rapid, automated and novel method is presented to extract DNA suitable for polymerase chain reaction (PCR) from small amounts of cereal leaf tissue in a high-throughput cost-effective way. The method uses a 96-well plate in which leaf samples are frozen, mechanically crushed using a matrix mill, macerated in alkali and subsequently neutralized. The method was used routinely with barley and wheat leaf samples and the extracted material was used to screen for specific traits of interests, including barley yellow dwarf virus resistance and β-amylase activity in barley and stem rust resistance in wheat. This system allows complete PCR analysis of 384 seedlings or more by one person in a day.

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Authors and Affiliations

  1. Western Australian State Agricultural Biotechnology Centre, Division of Science and Engineering, Murdoch University, 6150, Perth, WA, Australia

    Maxime Paris & Meredith Carter

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  1. Maxime Paris
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  2. Meredith Carter
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Correspondence to Maxime Paris.

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Paris, M., Carter, M. Cereal DNA: A rapid high-throughput extraction method for marker assisted selection. Plant Mol Biol Rep 18, 357–360 (2000). https://doi.org/10.1007/BF02825063

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  • DOI: https://doi.org/10.1007/BF02825063

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