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Use of radioactive—Labeled ABA for rapid determination of leaf abscission periods in cotton seedlings

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Abstract

A simple and rapid method is described for determination of cotton leaf abscission periods using radioactive-labeled ABA in cotton seedlings. A step-by-step protocol for cotton aged 5–6 days is provided.

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Abbreviations

ABA:

abscisic acid

dTT:

dithiohtreitol

References

  • Tursunkhodjaeva FM Azimova SS (1998) ABA—binding protein of cotton Gossipium hirsutum and specificity of its binding to ABA. J Chemistry of natural compounds 5: 688–690.

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  • Lowry OH, Rosenbrangh NJ, Farr AE and Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275.

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Authors and Affiliations

  1. Institute of Genetics and Plants Experimental Biology of Sciences Academy of the Republic of Uzbekistan, P.O.B. 97, 700000, Tashkent, Uzbekistan

    Firusa M. Tursunkhodjaeva, Jura A. Musaev & Shakhnosa S. Azimova

Authors
  1. Firusa M. Tursunkhodjaeva
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  2. Jura A. Musaev
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  3. Shakhnosa S. Azimova
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Correspondence to Firusa M. Tursunkhodjaeva.

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Tursunkhodjaeva, F.M., Musaev, J.A. & Azimova, S.S. Use of radioactive—Labeled ABA for rapid determination of leaf abscission periods in cotton seedlings. Plant Mol Biol Rep 18, 271–274 (2000). https://doi.org/10.1007/BF02823998

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  • DOI: https://doi.org/10.1007/BF02823998

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