Abstract
The form of contact seam (whether a continuous parallel seam or membranes in spatially periodic contact) has been characterized for normal and for neuraminidase pretreated human erythrocytes following adhesion in solutions of polylysine in the molecular mass range 10–225 kDa at concentrations from 0.5 to 1.0 mg/mL. The adhesion contact seam was spatially periodic for all normal control cells in polylysine. The lateral separation of contacts decreased from 1.6 to 0.8 μm as the concentration of 225 kDa polylysine was increased threefold from the adhesion threshold value. The separation distance did not change further even at high polymer concentrations that increased the electrophoretic velocity to positive values over twice the modulus of the velocity of control cells. The probability of cell adhesion decreased at these high polymer concentrations. The lateral contact separation increased and cell adhesion decreased for cells pretreated with neuraminidase. Cell adhesion did not occur when neuraminidase reduced the cell electrophoretic velocity modulus by 30%. Following neuraminidase pretreatments that allowed a small amount of adhesion, the cell contact seam was continuous rather than spatially peridic. The results show that a procedure that increases (e.g., polymer concentration increase) or decreases (e.g., enzyme removal of polycation crosslinking site) attraction leads to shorter (to a limiting value) or longer lateral contact separation, respectively.
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Katchalsky, A., Danon, D., Nevo, A., and de Vries, A. (1959)Biochim. Biophys. Acta 33, 120–138.
Marikovsky, Y., Danon, D., and Katchalsky, A. (1966)Biochim. Biophys. Acta 124, 154–159.
Coakley, W. T., Hewison, L. A., and Tilley, D. (1985)Eur. Biophys. J. 13, 123–130.
Hewison, L. A. (1988).Spatial Periodicity of Cell-Cell Contact: An Interfacial Instability Approach. Ph.D. Thesis, University of Wales, UK.
Hewison, L. A., Coakley, W. T., and Meyer, H. W. (1988)Cell Biophys. 13, 151–157.
Danon, D., Howe, C., and Lee, L. T. (1965)Biochim. Biophys. Acta 101, 201–213.
Darmani, H. and Coakley, W. T. (1990)Biochim Biophys. Acta 1021, 182–190.
Baker, A. J., Coakley, W. T., and Gallez, D. (1993)Eur. Biophys. J. 22, 53–62.
Dimitrov, D. S. and Jain, R. K. (1984)Biochim. Biophys. Acta 779, 437–468.
Gallez, D. and Coakley, W. T. (1986)Prog. Biophys. Mol. Biol. 48, 155–199.
Coakley, W. T. and Gallez, D. (1989)Biosci. Rep. 9, 675–691.
Sutherland, W. H. and Pritchard, J. A. V. (1979) inCell Electrophoresis: Clinical Applications and Methodology, Preece, A. W. and Sabolovic, D., eds., Elsevier/North Holland, Amsterdam, pp. 421–430.
Marra, J. and Hair, M. L. (1988)J. Phys. Chem. 92, 6044–6051.
Darmani, H., Coakley, W. T., Hann, A. C., and Brain, A. (1990)Cell Biophys. 16, 105–126.
Darmani, H. and Coakley, W. T. (1991)Cell Biophys. 18, 1–13.
Ginsburg, I., Mor, N., Resnick, M., and Bercovier, H. (1986)Eur. J. Cell Biol. 41, 130–133.
Nagura, H., Asai J., Katsumata, Y., and Kojima, K. (1973)Acta Path. Jap. 23, 279–290.
Preston, T. M. and King, C. A. (1978)J. Cell Sci. 34, 145–158.
King, C. A., Cooper, L., and Preston, T. M. (1983)Protoplasma 118, 10–18.
Dimitrov, D. S. (1982)Colloid Polymer Sci. 260, 1137–1144.
Gallez, D., Prevost, M., and Sanfeld, A. (1984)Colloids Surf. 10, 123–131.
Akay, G. (1982)Polymer Eng. Sci. 22, 798–804.
Lefebvre, D. R., Dillard, D. A., and Ward, T. C. (1989)J. Adhesion 27, 1–18.
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Thomas, N.E., Coakley, W.T. & Akay, G. The lateral separation of contacts on erythrocytes agglutinated by polylysine. Cell Biophysics 20, 125–147 (1992). https://doi.org/10.1007/BF02823654
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DOI: https://doi.org/10.1007/BF02823654