Abstract
The effect of various physico-chemical factors on production of intra- and extracellular phospholipase A1 bySalmonella newport was investigated. Maximum intracellular enzyme levels were observed when cells were grown in brain heart infusion broth, after 12 h of incubation at 37°C. Highest level of extracellular phospholipase A1, however, was seen in synthetic medium (pH 7.0) after 24 h of incubation at 37°C. Agitation during incubation had no effect on the intracellular enzyme synthesis but enhanced extracellular enzyme levels. Addition of surfactants to the growth media significantly decreased both intra- and extracellular phospholipase A1 production.
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Saxena, M., Gupta, J.K. & Vadehra, D.V. Factors affecting intra- and extracellular phospholipase A1 production bySalmonella newport . Folia Microbiol 34, 195–201 (1989). https://doi.org/10.1007/BF02821292
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DOI: https://doi.org/10.1007/BF02821292