A highly active and electrophoretically homogeneous enzyme with MM 31 kDa and specific lipase activity 1009 U/mg was isolated and purified from bacteria Pseudomonas aeruginosa A12 culture medium using ion-exchange and hydrophobic chromatography. The physicochemical properties of the isolated lipase were studied.
Similar content being viewed by others
References
J. Pliego, J. C. Mateos, J. Rodriguez, F. Valero, M. Baeza, R. Femat, R. Camacho, G. Sandoval, and E. J. Herrera-Lopez, Sensors, Switzerland, 2015.
S. Ulker, A. Ozel, A. Colak, and S. A. Karaoglu, Turk. J. Biol., 35, 543 (2011).
Y. Zhang, J. Liu, L. Qiu, Y. Shi, Z. Su, Y. Xia, and F. Li, Ann. Microbiol., 55 (2), 101 (2005).
X. H. Yang, C. H. Yin, S. Z. Fu, B. W. Wang, and T. W. Tan, Zhongguo Youzhi (China Oils Fats), 29 (7), 29 (2004).
E. Dalmau, J. L. Montesinos, M. Lotti, and C. Casas, Enzyme Microb. Technol., 26 (9–10), 657 (2000).
A. F. Yasser Refaat, Biotechnol. Lett., 24 (14), 1217 (2002).
D. Dai and L. Xia, Biotechnol. Prog., 21 (4), 1165 (2005).
F. Hasan, A. A. Shah, and A. Hameed, Ann. Microbiol., 56 (3), 247 (2006).
I. G. Sultanova and T. F. Zubenko, Uzb. Biol. Zh., No. 5, 71 (1976).
H. J. Wang, Z. Q. Mei, X. Z. Liu, Y. B. Huang, and Z. S. Piao, J. Jilin Univ., Sci. Ed., 40 (4), 430 (2002).
S. Longhi, F. Fusetti, T. Grandon, M. Lotti, M. Vanoni, and M. Alberghina, Biochim. Biophys. Acta, 1131, 227 (1992).
R. C. Chang, S. J. Chou, and J. F. Shaw, Biotechnol. Appl. Biochem., 19, 93 (1994).
A. Sanchez, P. Ferrer, A. Serrano, M. A. Pernas, F. Valero, M. L. Rua, C. Casas, and C. Sola, Enzyme Microb. Technol., 25 (3–5), 214 (1999).
S. Kanimozhi and K. Perinbam, Biomed. Pharmacol. J., 3 (2), 329 (2010).
O. H. Lowry, N. J. Rosenbrough, A. L. Farr, and R. J. Randall, J. Biol., Chem., 193, 265 (1951).
S. P. Borkar, G. R. Bodade, R. S. Rao, and C. N. Khobragade, Braz. J. Microbiol., 40 (2), 358 (2009).
Z. Benattouche, B. Abbouni, and B. R. Ghalem, Afr. J. Biotechnol., 11 (60), 12415 (2012).
C. C. Gaonkar, M. Keni, B. Banavath, T. Y. Mudaraddi, S. V. Hiremath, P. M. Biradar, and D. K. Veeranagoudar, Int. J. Adv. Sci. Res. Manage., 3 (3), 47 (2018).
U. K. Laemmli, Nature, 227, 680 (1970).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Khimiya Prirodnykh Soedinenii, No. 6, November−December, 2019, pp. 965−968.
Rights and permissions
About this article
Cite this article
Berdiev, N.S., Ziyavitdinov, Z.F., Bozorov, S.S. et al. Accumulation and Excretion Dynamics and Purification of Lipase from Bacteria Pseudomonas aeruginosa A12 Culture Medium. Chem Nat Compd 55, 1123–1126 (2019). https://doi.org/10.1007/s10600-019-02909-y
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10600-019-02909-y