Abstract
In addition to the general amino acid transport system (GAP) ofS. cerevisiae l-tryptophan is transported by another system with approximately 25% capacity of GAP, with aK T of 0.41±0.08 mmol/L and with a similar specificity as GAP (lower inhibition by Met, Pro, Ser, Thr and 2-aminoisobutyric acid; greater inhibition by Glu and His). The pH optimum of this system is at 5.0–5.5, activation energy above the transition point (20°C) was 20 kJ/mol, below the transition point 55 kJ/mol. The transport by this system was virtually unidirectional, efflux amounting to at most 10% into a tryptophan-free medium. The transport itself was blocked by 2,4-dinitrophenol, antimycin A and uranyl nitrate. The system was synthesized de novo during preincubation with glucose=fructose>trehalose >ethanol within 30 min, and was degraded with a half-time of 15 min in the absence of further synthesis. The accumulation ratios ofl-tryptophan ingap1 mutants were concentration-dependent (200∶1 at 1 μmoll-Trp/L, 4∶1 at 2.5 mmoll-Trp/L) and decreased with increasing suspension density from 200∶1 to 5∶1 (for 10 μmoll-Trp/L). The involvement of hydrogen ions in the uptake was clearly demonstrated by the effect of D2O even if it could not be established by either shifts of pHout or membrane depolarization.
Similar content being viewed by others
References
Crabeel M., Grenson M.: Regulation of histidine uptake by specific feedback inhibition of two histidine permeases inSaccharomyces cerevisiae.Eur. J. Biochem. 14, 197–204 (1970).
García J.C., Kotyk A.: Effect of ethanol on the specific transport system forl-lysine inSaccharomyces cerevisiae.Folia Microbiol. 33, 281–284 (1988a).
García J.C., Kotyk A.: Uptake ofl-lysine by a double mutant ofSaccharomyces cerevisiae.Folia Microbiol. 33, 285–291 (1988b).
Grenson M., Hou C., Crabeel M.: Multiplicity of the amino acid permeases inSaccharomyces cerevisiae. IV. Evidence for a general amino acid permease.J. Bacteriol. 103, 770–777 (1970).
Halvorson H.O., Cohen G.N.: Incorporation des aminoacides endogènes et exogènes dans les protéines de la levure.Ann. Inst. Pasteur 95, 83–87 (1958).
Hauer R., Höfer M.: Evidence for interactions between the energy-dependent transport of sugars and the membrane potential in the yeastRhodotorula gracilis (Rhodosporidium toruloides).J. Membr. Biol. 43, 335–349 (1978).
Horák J.: Amino acid transport in eucaryotic microorganisms.Biochim. Biophys. Acta 864, 223–256 (1987).
Horák J., Kotyk A., Říhová L.: Specificity oftrans-inhibition of amino acid transport in baker's yeast.Folia Microbiol. 22, 360–362 (1977).
Kotyk A., Říhová L.: Transport of α-aminoisobutyric acid inSaccharomyces cerevisiae. Feedback control.Biochim. Biophys. Acta 288, 380–389 (1972).
Kotyk A., Stružinský R.: Effect of high substrate concentrations on active transport parameters.Biochim. Biophys. Acta 470, 484–491 (1977).
Kotyk A., Michaljaničová D.: Suspension density and accumulation ratio of sugars and amino acids in yeasts.Folia Microbiol. 32, 459–464 (1987).
Kotyk A., Horák J., Knotková A.: Transport protein synthesis in nongrowing yeast cells.Biochim. Biophys. Acta 698, 243–251 (1982).
Kotyk A., Dvořáková M., Koryta J.: Deuterons cannot replace protons in active transport processes in yeast.FEBS Lett. 264, 203–205 (1990).
Opekarová M., Kotyk A., Horák J., Kholodenko V. P.: Isolation and properties of an arginine-binding protein fromSaccharomyces cerevisiae Eur. J. Biochem. 59, 373–376 (1975).
Slavík J.: Intracellular pH of yeast cells measured with fluorescent probes.FEBS Lett. 140, 22–26 (1982).
Taylor E. S.: The assimilation of amino acids by bacteria. III. Concentration of free amino acids in the internals environment of various bacteria and yeasts.J. Gen. Microbiol. 1, 86–90 (1947).
Theuvenet A.P.R., Borst-Pauwels G.W.F.H.: The influence of surface charge on the kinetics of ion translocation across biological membranes.J. Theor. Biol. 57, 313–329 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kotyk, A., Dvořáková, M. Transport ofl-tryptophan inSaccharomyces cerevisiae . Folia Microbiol 35, 209–217 (1990). https://doi.org/10.1007/BF02820487
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02820487