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Effects of the Fenton reagent on transport in yeast

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Abstract

In the facultatively anaerobic yeastSaccharomyces cerevisiae the uptake rate and the accumulation ratio of 2-aminoisobutyric acid was decreased by some 30% by Fenton's reagent (FR), a powerful source of OH… radicals. Likewise, the uptake of glutamic acid, leucine and arginine was diminished. The mediated diffusion of 6-deoxy-d-glucose was not affected. The H+ symport of maltose and trehalose was inhibited by some 40% both in the initial rate and in the accumulation ratio. FR had a dramatic inhibitory effect when present during preincubation with 50 mmol/L glucose. In the obligately aerobicLodderomyces elongisporus the uptake of all amino acids tested was decreased by 15–30%, that of 6-deoxy-d-glucose by about 10%. The initial rates of uptake of maltose and trehalose were depressed by FR by 40% and the acceleration of uptake observed after 8 min of incubation, was abolished by FR completely. Acidification rate of the external medium byS. cerevisiae in the presence of glucose or galactose was enhanced three-fold, that after subsequently added K+ was substantially decreased. FR appears to have a dual effect on sugar and amino acid transport processes in yeast: (1) it blocks carrier protein synthesis, (2) it inhibits the source of energy for transport. It does not appreciably affect the carrier proteins themselves.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, University of Chiang Mai, 50200, Chiang Mai, Thailand

    U. Khansuwan

  2. Department of Membrane Transport, Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20, Prague, Czechia

    A. Kotyk

Authors
  1. U. Khansuwan
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  2. A. Kotyk
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Khansuwan, U., Kotyk, A. Effects of the Fenton reagent on transport in yeast. Folia Microbiol 45, 515–520 (2000). https://doi.org/10.1007/BF02818720

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