Abstract
Soil fertilization with urea at rates of 0.2 and 0.5 mg N/g soil was toxic for total counts of bacteria and fungi except with cellulolytic fungi where growth was promoted by addition of urea after 90-d incubation. Also, the population numbers of both bacteria and fungi were significantly decreased when soil was amended with CaCl2 and K2SO4 applied at two levels (50 and 100 μmol/g soil). Some alleviation of the toxic effect of either urea or the inorganic salts was observed when they were applied in combination. Fungal species composition was found to be affected in the treated soil. The most tolerant fungi wereAlternaria alternata, Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Eurotium amstelodami, E. chevalieri, Nectria hœmatococca, Pœcilomyces variotii andStachybotrys chartarum. Other species of fungi were either sensitive to all treatments or sensitive to some and tolerant to others. Ammonium nitrogen was found to be more accumulated in soil treated with urea mixed with inorganic salts compared with soil treated only with urea whereas NO −3 -N (and NO −2 -N) was decreased. The overall effect of the addition of inorganic salts on total mineralized nitrogen, however was promotive. Soil pH was increased in ureatreated soil but was depressed by application of CaCl2 or K2SO4 to values lower than that of untreated soil. The results may be of agronomical interest in overcoming problems encountered with urea application as N fertilizer.
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References
Alexander I.J., Fairley R.I.: Effects of N fertilization on populations of fine roots and mycorrhizas in spruce humus.Plant & Soil71, 49–53 (1983).
Allen O.N.:Experiments in Soil Bacteriology, pp. 11–12 (O.N. Allen, Ed.). Burgess Publ. Co., Minneapolis 1949.
Arnebrant K., Bååth E., Söderström B.: Changes in microfungal community structure after fertilization of scots pine forest soil with ammonium nitrate or urea.Soil Biol. Biochem.22, 309–312 (1990).
Bååth E., Lundgreen B., Söderström B.: Effects of nitrogen fertilization on the activity and biomass of fungi and bacteria in a podzolic soil.Z. Bakteriol. Mikrobiol. Hyg., I. Abt. Orig. C 2, 90–98 (1981).
Bache B.W.: Determination of pH, lime potential, and aluminium hydroxide potential of acid soils.J. Soil Sci.20, 28–37 (1970).
Banerjee A., Banerjee A.K.: Influence of captan on some microorganisms and microbial processes related to the nitrogen cycle.Plant & Soil102, 239–245 (1978).
Blum U., Shafer S.R.: Microbial populations and phenolic acid in soil.Soil Biol. Biochem.20, 793–800 (1988).
Booth C.:The Genus Fusarium, p. 237. Commonwealth Mycological Institute Kew (Surrey, UK) 1971.
Booth C.:Fusarium: Laboratory Guide to the Identification of the Major Species, p. 58. Commonwealth Mycological Institute, Kew (Surrey, UK) 1977.
Broadbent F.E.: Effect of fertilizer nitrogen on the release of soil nitrogen.Soil Sci. Soc. Amer. Proc.29, 692–696 (1965).
Campino I.: The effect of superphosphate and potassium fertilizer and salts on the nitrogen mineralization of incubated meadow soil.Fertil. Res.3, 325–336 (1982).
Domsch K.H., Gams W., Anderson T.H.:Compendium of Soil Fungi. Academic Press, New York (NY) 1980.
Ellis M.B.:Dematiaceous Hyphomycetes, p. 608. Commonwealth Mycological Institute, Kew (Surrey, UK) 1971.
Ellis M.B.:More Dematiaceous Hyphomycetes, p. 507. Commonwealth Mycological Institute, Kew (Surrey, UK) 1976.
El-Shinnawi M.M.: Salts affecting microorganisms in certain soils.Z. Bakteriol. Parasitenk. Infekt. Krankh. Hyg., Abt. II 130, 387–394 (1975).
Engelstad O.P., Hauck R.D.: Urea: will it become the most popular nitrogen carrier?Crops & Soils26, 11–14 (1974).
Filter A.H., Hay R.K.:Environmental Physiology of Plants. Academic Press, London 1987.
Frostegärd A., Tunlid A., Bååth E.: Changes in microbial community structure during long-term incubation in two soils experimentally contaminated with metals.Soil Biol. Biochem.28, 55–63 (1996).
Garbaye J., Le Tacon F.: Influence of mineral fertilization and thinning intensity on the fruit body production of epigeous fungi in an artificial spurce stand (Picea excelsaLink) in north-eastern France.Acta Ecol./Ecol. Plant.3, 153–160 (1982).
Gasser J.K.R.: Urea as a fertilizer.Soils & Fertil.27, 175–180 (1964).
Golden D.C., Sivasubrananiam S., Sandanam S., Wijedasa M.A.: Inhibitory effects of commercial potassium chloride on the nitrification rates of added ammonium sulphate in an acid red yellow podzolic soil.Plant & Soil59, 147–151 (1981).
Heilman P.: Effect of added salts on nitrogen release and nitrate levels in forest soils of the Washington costal area.Soil Sci. Soc. Amer. Proc.39, 778–782 (1975).
Hemida S.K., Omar S.A., Abdel-Mallek A.Y.: Microbial populations and enzyme activity in soil treated with heavy metals.Water Air Soil Pollut.95, 13–22 (1997).
Johnson L.F., Curl E.A.:Methods for Research on Ecology of Soil-borne Pathogens, p. 247. Burgess Publ. Co., Minneapolis 1972.
Jones J.M., Richards B.N.: Effect of reforestation on turnover of15N-labelled nitrate and ammonium in relation to changes in soil microflora.Soil Biol. Biochem.9, 383–392 (1977a).
Jones J.M., Richards B.N.: Changes in the microbiology of eucalypt forest soils following reforestation with exotic pines.Austral. Forest. Res.7, 229–240 (1977b).
Kozakiewicz Z.:Aspergillus Species on Stored Products. C.A.B. International Mycological Institute, Mycological Papers no. 161, p. 188 (1989).
Laiho O., Srrjala T., Hyvarinen R., Rautiainen L.: Effect of fertilization on mycorrhizae in pine stands.Folia Forest. 699 (1987).
Lewis D.H.: Comparative aspects of the carbon nutrition of mycorrhizas, pp. 119–148 inEndomycorrhizas (F.E. Sanders, B. Mosse, P.B. Tinker, Eds). Academic Press, London 1975.
Malik K.A.: Biological methods of reclamation of salt affected soil, pp. 105–109 inTechnology for Increasing Food Production (J.C. Holmes, Ed.). Proc. 2nd FAO/SIDA Seminar on Field Food Crops in Africa and Near East, Lahore (Pakistan) 1977.
Malik K.a., Bhatti N.A., Kauser F.O.: Effect of soil salinity on decomposition and humification of organic matter by some cellulolytic fungi.Mycologia71, 811–820 (1979).
Martikainen P.J.: Nitrification in forest soil of different pH as affected by urea, ammonium sulphate and potassium sulphate.Soil Biol. Biochem.17, 363–367 (1985).
McCormick R.W., Wolf D.C.: Effect of sodium chloride on CO2 evolution, ammonification, and nitrification in a Sassafras sandy loam.Soil Biol. Biochem.12, 153–157 (1980).
Omar S.A., Abdel-Sater M.A., Khallil A.M., Abd-Alla M.H.: Growth and enzyme activities of fungi and bacteria in soil salinized with sodium chloride.Folia Microbiol.39, 23–28 (1994).
Pitt J.I.:The Genus Penicillium and Its Teleomorphic States Eupenicillium and Talaromyces. Academic Press, London 1979.
Raper K.B., Fennell D.I.:The Genus Aspergillus. Williams and Wilkins, Baltimore 1965.
Sahrawat K.L., Prasad R.: A rapid method for determination of nitrate, nitrite and ammonical nitrogen in soils.Plant & Soil42, 303–308 (1975).
Schofield R.K., Taylor A.W.: The measurement of soil pH.Soil Sci. Soc. Am. Proc.19, 164–167 (1955).
Sivanesan A.:The Bitunicate Ascomycetes and Their Anamorphs. Strauss and Cramer GmbH, Germany 1984.
Sivanesan A.:Graminicolous Species of Bipolaris, Curvularia, Drechslera, Exserohilum and Their Teleomorphs. C.A.B. International Mycological Institute, Mycological Paper no. 158, p. 261 (1987).
Smith N.R., Dawson V.T.: The bacteriostatic action of rose bengal in media used for the plate count of soil fungi.Soil Sci.58, 467–471 (1944).
Snell F.D., Snell C.T.:Colorimetric Methods of Analysis. II. Van Nostrand, New York 1949.
Thom C., Raper K.B.:A Manual of the Aspergilli. Williams and Wilkins, Baltimore 1945.
Tomlinson T.E.: Urea: agronomic applications.Proc. Fertil. Soc. II3, 1–76 (1970).
Turner S.M., Newman E.I., Campbell R.: Microbial population of ryegrass root surface: influence of nitrogen and phosphorus supply.Soil Biol. Biochem.17, 711–715 (1985).
van Vuurde J.W.L.: The rhizosphere microflora of wheat grown under controlled conditions. I. The effect of soil fertility and urea leaf treatment on the rhizoplane microflora.Plant & Soil50, 447–460 (1978).
van Vuurde J.W.L., de Lange A.: The rhizosphere microflora of wheat grown under controlled conditions. II. Influence of the stage of growth of the plant, soil fertility and leaf treatment with urea on the rhizosphere soil microflora.Plant & Soil50, 461–472 (1978).
Vraný J.: Respiration and microflora of the rhizosphere of wheat influenced by foliar application of urea.Folia Microbiol.26, 118–123 (1983).
Wollum IIA.G., Davey C.B.: Nitrogen accumulation, transformation and transport in forest soils, pp. 67–106 inForest, Soils and Forest Land Management (B. Bernier, C.H. Winget, Eds). Proc. 4th North America Forest Soils Conference. Los Presses de l'Universite Laval, Quebec 1975.
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Omar, S.A., Ismail, M.A. Microbial populations, ammonification and nitrification in soil treated with urea and inorganic salts. Folia Microbiol 44, 205–212 (1999). https://doi.org/10.1007/BF02816244
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DOI: https://doi.org/10.1007/BF02816244