Abstract
For successful transformation of prototrophic industrial yeast strains dominant selectable markers are necessary. In the present study we show the applicability of a selection system based on the phenotype of multidrug resistance. The mutantpdr 3–9 allele on centromeric or episomal vector, encoding a more efficient transcriptional activator with Y276H amino acid substitution, was used as a dominant selectable marker for selection of transformants. Thepdr3–9 allele conferred resistance of transformed cells to cycloheximide, chloramphenicol, mucidin and oligomycin both in the absence and in the presence of a chromosomal copy of thePDR3 gene. Both multicopy YEp352/pdr3–9 and centromeric pFL38/pdr3–9 vectors bearing the mutantpdr3–9 allele have proved to be a valuable tool for a direct selection of transformants of industrial strains ofSaccharomyces cerevisiae.
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Lacková, D., Šubík, J. Use of mutatedPDR3 gene as a dominant selectable marker in transformation of prototrophic yeast strains. Folia Microbiol 44, 171–176 (1999). https://doi.org/10.1007/BF02816237
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DOI: https://doi.org/10.1007/BF02816237