Skip to main content
SpringerLink
Log in

A mutated ARO4 gene for feedback-resistant DAHP synthase which causes both o-fluoro-dl-phenylalamine resistance and β-phenethyl-alcohol overproduction in Saccharomyces cerevisiae

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

o-Fluoro-dl-phenylalanine (OFP)-resistant mutants which overproduce β-phenethyl-alcohol were isolated from a laboratory strain of Saccharomyces cerevisiae. Cells of one of the mutants accumulated tyrosine and phenylalanine 1.5–3 fold more than did wild-type cells. Its 3-deoxy-d-arabino-hepturosonate-7-phosphate (DAHP) synthase (EC 4.1.2.15), encoded by ARO4, was free from feedback inhibition by tyrosine. Genetic analysis revealed that the mutation was controlled by a single dominant gene, ARO4-OFP, encoding feedback-resistant DAHP synthase by tyrosine, and that this gene caused both the OFP resistance and β-phenethyl-alcohol overproduction. This was supported by molecular genetic studies using cloned ARO4 both from the wild-type and its mutant strain.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Äyräpää T (1965) J Inst Brew 71:341–347

    Google Scholar 

  • Fukuda K, Watanabe M, Asano K, Ueda H, Ohta S (1990a) Agri Biol Chem 54:269–271

    Google Scholar 

  • Fukuda K, Watanabe M, Asano K (1990b) Agri Biol Chem 54:3151–3156

    Google Scholar 

  • Fukuda K, Watanabe M, Asano K, Ouch K, Takasawa S (1991) Curr Genet 20:449–452

    Google Scholar 

  • Ito H, Sato K, Matsui K, Sano K, Nakamori S, Tanaka T (1990) Agri Biol Chem 54:707–713

    Google Scholar 

  • Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168

    Google Scholar 

  • Lingens F, Goebel W, Uesseler H (1967) Eur J Biochem 1:363–374

    Google Scholar 

  • Meuris P (1973) Genetics 76:735–744

    Google Scholar 

  • Paravicini G (1989) A dissertation submitted to the Swiss federal institute of technology Zurich for the degree of doctor of natural sciences

  • Spencer JFT, Spencer DM (1988) In: Campbell I, Duffus JH (eds) Yeast: a practical approach. IRL Press, Oxford, pp 65–106

    Google Scholar 

  • Takahashi M, Chan W (1971) Can J Biochem 49:1015–1025

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Foods and Liquors Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., 4041 Ami, Inashiki, 300-03, Ibaraki, Japan

    Kazuro Fukuda, Makoto Watanabe, Kozo Asano & Seigo Takasawa

  2. Tokyo Research Laboratories, Kyowa Hakko Kogyo Co. Ltd., Machida, 194, Tokyo, Japan

    Kozo Ouchi

Authors
  1. Kazuro Fukuda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Makoto Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kozo Asano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kozo Ouchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seigo Takasawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by B. S. Cox

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fukuda, K., Watanabe, M., Asano, K. et al. A mutated ARO4 gene for feedback-resistant DAHP synthase which causes both o-fluoro-dl-phenylalamine resistance and β-phenethyl-alcohol overproduction in Saccharomyces cerevisiae . Curr Genet 20, 453–456 (1991). https://doi.org/10.1007/BF00334771

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00334771

Key words

Search

Navigation