Abstract
The stem nodules of the legumeAeschynomene aspera contain indoleacetic acid and a high amount of tryptophan. TheAzorhizobium caulinodans isolated from the stem nodules of the leguminous emergent hydrophyte produced a high amount of IAA (14.8 mg/L) inl-tryptophan-supplemented basal medium. The IAA yields paralleled the culture growth rate and increased up to 52 h. No separate growth and production phase was observed. The IAA production was increased 344% when the medium was supplemented withl-tryptophan, sucrose, FeSO4·7H2O, NaNO3, ascorbic acid and sodium dodecyl sulfate. The possible role of the IAA production in the legume-bacterium symbiosis is discussed.
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Beltra R., Diaz F., Fraile G.: The formation of growth substances byRhizobium species.Zentr. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2. Naturwiss. Mikrobiol. Landwirtsch. Technol. Umweltsch.135, 617–622 (1980).
Bahttacharyya R.N., Basu P.S.: Studies of the root nodules of leguminous plants. IV. Production of indole acetic acid by aBradyrhizobium sp. from the root nodules of a leguminous shrubCrotalaria retusa L.Acta Biotechnol.11, 439–447 (1991).
Bhattacharyya R.N., Basu P.S.: Bioproduction of indole acetic acid by aRhizobium sp. from root nodules of a leguminous climber,Psophocarpus tetragonolobus DC.Ind. J. Exp. Biol.30, 632–635 (1992).
Bhowmick P.K., Basu P.S.: Indole acetic acid production byRhizobium sp. from a leguminous treeSesbania grandifloraPers.Egypt. J. Microbiol.22, 293–301 (1987).
Chattopadhyay K.K., Basu P.S.: Bioproduction of indole acetic acid by theRhizobium sp. from root nodules of a leguminous treeDalbergia sissooRoxe.Acta. Microbiol. Polon.38, 293–305 (1989).
Clark A.G.: Indole acetic acid proeduction byAgrobacterium andRhizobium sp.Microbios11A (46), 29–35 (1974).
Conn H.J., Jennison M.W., Weeks O.B.: Routine tests for the identification of bacteria, pp. 140–168 in H.J. Conn (Ed.):Manual of Microbiological Methods. McGraw-Hill Book Co., New York 1957.
De Bruijn F.J.: The unusual symbiosis between the diazotrophic stem-nodulating bacteriumAzorhizobium caulinodans ORS 571 and its host, the tropical legumeSesbania rostrata, pp. 457–504 in T. Kosuge, E.W. Nester (Eds):Plant-Microbe Interaction (Molecular and Genetic Perspectives), Vol. 3. McGraw-Hill Publ. Co., New York 1989.
Dreyfus B., Garcia J.L., Gillis M.: Characterization ofAzorhizobium caulinodans gen.nov. sp.nov., a stem-nodulating nitrogen-fixing bacterium isolated fromSesbania rostrata.Int. J. Syst. Bacteriol.38, 89–98 (1988).
Dullaart J.: Quantitative estimation of indoleacetic acid and indolecarboxylic acid in root nodules and roots ofLupinus luteus L.Acta Bot. Neerl.16, 222–230 (1967).
Gordon S.A., Weber R.P.: Colorimetric estimation of indoleacetic acid.Plant Physiol.26, 192–195 (1951).
Garcia-Rodriguez T., Gutierrez-Navarroa M., Jimenez R., Perez-Silva J.: Effects of legume root exudates on indoleacetic acid production byRhizobium meliloti.Polish J. Soil Sci.14, 45–52 (1981).
Hamilton R.H., Bandurski R.S., Grisby B.H.: Isolation of indole-3-acetic acid from corn kernels and etiolated corn seedlings.Plant Physiol.36, 354–359 (1961).
Hassan S.S.M.: Spectrophotometric method for simultaneous determination of tryptophan and tyrosine.Anal. Chem.47, 1429–1432 (1975).
Jordan D.C.: Rhizobiaceae, pp. 234–244 in N.R. Krieg, J.G. Holt (Eds):Bergey's Manual of Systematic Bacteriology, Vol. 1. Williams & Wilkins, Baltimore 1984.
Markova A., Chanova D.: Effects of molybdenum and cobalt on nitrogen fixing activity ofRhizobium japonicum and soybean yield.Task. Veg. Sci.13, 187–190 (1984).
Moore T.C.:Biochemistry and Physiology of Plant Hormones, 2nd ed. Springer-Verlag, New York 1989.
Nitsch J.P.: Free auxin and free tryptophan in strawberry.Plant Physiol.30, 33–39 (1955).
Panse V.G., Sukhatme P.V.:Statistical Methods for Agricultural Workers, 4th ed., pp. 145–156. Indian Council of Agricultural Research, New Delhi 1985.
Sinha B.K., Basu P.S.: Indole-3-acetic acid and its metabolism in root nodules ofPongamia pinnata (L.)Pierre.Biochem. Physiol. Pflanzen176, 218–227 (1981).
Skerman V.B.D.:A Guide to Identification of the Genera of Bacteria, pp. 189–191. Williams & Wilkins, Baltimore 1959.
Stoessl A., Venis M.A.: Determination of submicrogram levels of indole-3-acetic acid: A new, highly specific method.Anal. Biochem.34, 344–351 (1970).
Vincent J.M.: Root nodule symbiosis withRhizobium, pp. 265–341 in A. Quespel (Ed.):The Biology of Nitrogen Fixation. North-Holland Publ. Co., Amsterdam 1974.
Wheeler C.T., Henson I.E., McLaughlin M.E.: Hormones in plant bearing actinomycete root nodules.Bot. Gaz. (Suppl.)140, S52-S57 (1979).
Yoshida S., Yatazawa M.: Species characteristics and cultural conditions as affecting to rhizobial production of IAA.Nippon Dojo Hiryogaku Zasshi44, 63–66 (1973).
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Ghosh, A.C., Basu, P.S. Culture growth and IAA production by a microbial diazotropic symbiont of stem-nodules of the legumeAeschynomene aspera . Folia Microbiol 42, 595–600 (1997). https://doi.org/10.1007/BF02815472
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DOI: https://doi.org/10.1007/BF02815472