Skip to main content
SpringerLink
Log in

Variability of ligninolytic enzyme activities in basidiospore isolates of the fungusPleurotus ostreatus in comparison with that of protoplast-derived isolates

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Variability of production of all tested ligninolytic enzymes (laccase, peroxidase and manganese-dependent peroxidase) was substantially higher in isolates derived from basidiospores of strain F6 of the white-rot basidiomycetePleurotus ostreatus than the relatively low variability of the dikaryotic mycelial colonies of this strain, and also higher than the variability of protoplast-derived isolates of the same strain. The difference was caused mainly but not completely by the monokaryotic nature of the isolates. To reach the best fructification of the fungus, two fructification media were chosen from twenty two tested and a modified technique of fructification was used.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bourbonnais R., Paice M.G.: Oxidation of non-phenolic substrates. An expanded role for laccase in lignin biodegradation.FEBS Lett. 267, 99–102 (1990).

    Article  PubMed  CAS  Google Scholar 

  • Challen M.P., Elliott T.J.: An alternative vessel for thein vitro fruiting of macromycetes.Bull. Brit. Mycol. Soc. 19, 135–137 (1985).

    Article  Google Scholar 

  • Eger G.: Biology and breeding ofPleurotus, pp. 497–519 in S.T. Chang, W.A. Hayes (Eds):The Biology and Cultivation of Edible Mushrooms. Academic Press, London 1978.

    Google Scholar 

  • Eger G.: Untersuchungen über die Bildung und Regeneration von Fruchtkörpern bei Hutpilzen. I.Pleurotus florida.Arch. Mikrobiol. 50, 343–356 (1965).

    Article  PubMed  CAS  Google Scholar 

  • Eriksson K.-E.L., Blanchette R.A., Ander P.:Microbial and Enzymatic Degradation of Wood and Wood Components. Springer-Verlag, Berlin-Heidelberg 1990.

    Google Scholar 

  • Gold M.H., Cheng T.M.: Conditions for fruit body formation in the white rot basidiomycetePhanerochœte chrysosporium.Arch. Microbiol. 121, 37–41 (1979).

    Article  CAS  Google Scholar 

  • Homolka L., Paltiel J., Voláková I., Nerud F., Hadar Y.: The effect of growth conditions and genetic background on laccase production in the fungusPleurotus ostreatus.Folia Microbiol. 42, 527–529 (1997).

    CAS  Google Scholar 

  • Homolka L., Voláková I., Nerud F.: Variability of enzymatic activities in lignolytic fungiPleurotus ostreatus andLentinus tigrinus after protoplasting and UV-mutagenization.Biotechnol. Techn. 9, 157–162 (1995).

    Article  CAS  Google Scholar 

  • Jablonský I., Srb A., Šašek V.:Cultivation of Edible Mushrooms. (In Czech) SZN, Prague 1985.

    Google Scholar 

  • Keller U.: Highly efficient mutagenesis ofClaviceps purpurea by using protoplasts.Appl. Environ. Microbiol. 46, 580–584 (1983).

    PubMed  Google Scholar 

  • Matsumoto T., Fukumasa-Nakai Y., Komatsu M.: Efficient dedikaryotization of higher basidiomycetes by the protoplast regeneration method.Rep. Tottori Mycol. Inst. 33, 29–33 (1995).

    Google Scholar 

  • Musílek V., Černá J., Šašek V., Semerdžieva M., Vondráček M.: Antifungal antibiotic of the basidiomyceteOudemansiella mucida. I. Isolation and cultivation of a producing strain.Folia Microbiol. 14, 377–387 (1969).

    Google Scholar 

  • Ngo T.T., Lenhoff H.M.: A sensitive and versatile chromogenic assay for peroxidase and peroxidase-coupled reactions.Anal. Biochem. 105, 389–397 (1980).

    Article  PubMed  CAS  Google Scholar 

  • Plunkett B.E.: The influence of factors of the aeration complex and light upon fruit-body form in pure cultures of anAgaric andPolypore.Ann. Bot. 20, 563–586 (1956).

    Google Scholar 

  • Scheld H.W., Perry J.J.: Studies on the formation of fruiting bodies in the basidiomyceteLenzites sœpiaria.J. Gen. Appl. Microbiol. 17, 199–205 (1971).

    Google Scholar 

  • Tien M., Kirk T.K.: Lignin peroxidase ofPhanerochœte chrysosporium.Methods Enzymol. 161B, 238–248 (1988).

    Article  Google Scholar 

  • Wessels J.G.H.: Fruiting in the higher fungi.Adv. Microb. Physiol. 34, 147–202 (1993).

    PubMed  CAS  Google Scholar 

  • Yoo Y.B., Song M.T., Go S.J., You C.H., Cha D.Y., Park Y.H., Chang K.Y.: Interorder protoplast fusion betweenPleurotus ostreatus andGanoderma applanatum.Kor. J. Mycol. 17, 119–123 (1989).

    CAS  Google Scholar 

  • Yurchenco J.A., Warren G.H.: A laboratory procedure for the cultivation and fructification of species ofHericium.Mycologia 53, 566–574 (1961).

    Article  Google Scholar 

  • Zadrazil F.: Cultivation ofPleurotus ostreatus, pp. 521–557 in S.T. Chang, W.A. Hayes (Eds):The Biology and Cultivation of Edible Mushrooms. Academic Press, London 1978.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague 4

    I. Eichlerová-Voláková & L. Homolka

Authors
  1. I. Eichlerová-Voláková
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Homolka
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Eichlerová-Voláková, I., Homolka, L. Variability of ligninolytic enzyme activities in basidiospore isolates of the fungusPleurotus ostreatus in comparison with that of protoplast-derived isolates. Folia Microbiol 42, 583–588 (1997). https://doi.org/10.1007/BF02815470

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02815470

Keywords

Search

Navigation