Molecular and Chemical Neuropathology

, Volume 23, Issue 2, pp 115–124

Freed-amino acids in human cerebrospinal fluid of alzheimer disease, multiple sclerosis, and healthy control subjects

  • George H. Fisher
  • Leonard Petrucelli
  • Christina Gardner
  • Carolyn Emory
  • William H. Frey
  • Luigi Amaducci
  • Sandro Sorbi
  • Giovanna Sorrentino
  • Mauro Borghi
  • Antimo D'aniello
Article

DOI: 10.1007/BF02815405

Cite this article as:
Fisher, G.H., Petrucelli, L., Gardner, C. et al. Molecular and Chemical Neuropathology (1994) 23: 115. doi:10.1007/BF02815405

Abstract

This is the first report of the presence of freeD-amino acids in lumbar and ventricular human cerebrospinal fluid (CSF) of individuals with Alzheimer disease (AD) compared with CSF of normal control subjects and with individuals affected by multiple sclerosis, as an unrelated neurologic disorder. Freed-amino acids are present at significantly higher levels in AD CSF than normal CSF, whereas in the CSF of patients affected by multiple sclerosis,d-amino acids occurs at the same level as in the normal controls. The totald-amino acid content in ventricular CSF was 1.48 times higher in the AD than controls (26.4 vs 17.9 nmol/mL,p=0.025). The totald-amino acid content was 1.43 times higher in AD lumbar CSF than controls (1.89 vs. 1.32 nmol/mL,p=0.001).d-Aspartate in particular was 2.74 times higher in AD ventricular CSF compared to normal ventricular CSF (3.34 vs 1.22 nmol/mL,p=0.029). In lumbar CSF,d-aspartate was 1.5 times higher in AD than controls (0.054 vs 0.036 nmol/mL,p=0.041). Previously we reported thatd-amino acids are elevated in AD brain proteins associated with neurofibrillary tangles compared to normal brain proteins (D'Aniello et al., 1992c; Fisher et al., 1992a,b). Thus, thed-amino acids present in CSF may originate from degradation of brain proteins.

Index Entries

d-Amino acids cerebrospinal fluid ventricular CSF lumbar CSF Alzheimer disease multiple sclerosis aspartic acid 

Copyright information

© Humana Press Inc 1994

Authors and Affiliations

  • George H. Fisher
    • 1
  • Leonard Petrucelli
    • 1
  • Christina Gardner
    • 1
  • Carolyn Emory
    • 2
  • William H. Frey
    • 2
  • Luigi Amaducci
    • 3
  • Sandro Sorbi
    • 3
  • Giovanna Sorrentino
    • 3
  • Mauro Borghi
    • 4
  • Antimo D'aniello
    • 5
  1. 1.Department of ChemistryBarry UniversityMiami Shores
  2. 2.Alzheimer's Treatment and Research Center Ramsey ClinicSt. Paul-Ramsey Medical CenterSt. Paul
  3. 3.Departimento di Scienze Neurologiche e PsichiatricheUniversity of FlorenceFlorenceItaly
  4. 4.Department of Medical Jurisprudence Policlinico Le ScotteUniversity of SienaItaly
  5. 5.Department of Biochemistry and Molecular BiologyStazione Zoologica ‘A Dohrn’NaplesItaly

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