Abstract
The autolyzates of three different strains ofFrancisella tularensis 15L, 130 and SCHU were tested for their immunogenic potential and protein heterogeneity. The autolyzates induce the production of specific antibodies, the delayed type of hypersensitivity, and some degree of protection against European virulent strain 130. This material (as antigen) was especially suitable for ELISA. When the autolyzates were subjected to SDS gradient PAGE, a variety of polypeptides were distinguished. The composition of polypeptides from all three strains on SDS-PAGE was almost identical. After the detection of antigenic determinants by Western blotting more than twenty bands appeared. The visualization with polyclonal antisera against live laboratory strain 15L and against autolyzates 15L, 130 and SCHU revealed differences in the composition of the antigenic determinants of these strains.
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References
Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal. Biochem. 72, 248–254 (1976).
Burke D. S.: Immunization against tularemia: Analysis of the effectiveness of liveFrancisella tularensis vaccine in prevention of laboratory-acquired tularemia.J. Infect. Dis. 135, 55–60 (1977).
Carlsson H.E., Lindberg A.A., Lindberg G., Hederstedt B., Karlsson K.A., Agell B.O.: Enzyme-linked immunosorbent assay for immunological diagnosis of human tularemia.J. Clin. Microbiol. 10, 615–621 (1979).
Claflin J.L., Larson C.L.: Infection-immunity in tularemia: Specificity of cellular immunity.Infect. Immun. 5, 311–318 (1972).
Holm S.E., Tärnvik A., Sandström G.: Antigen composition of a vaccine strain ofFrancisella tularensis.Int. Arch. Allergy Appl. Immun. 61, 136–144 (1980).
Karttunen R., Andersson G., Ekre H.P.T., Juutinen K., Surcel H.M., Syrjälä H., Herva E.: Interleukin 2 and gamma interferon production, interleukin 2 receptor expression, and DNA synthesis induced by tularemia antigenin vitro after natural infection or vaccination.J. Clin. Microbiol. 25, 1074–1078 (1987).
Koskela P., Herva E.: Cell-mediated and humoral immunity induced by a liveFrancisella tularensis vaccine.Infect. Immun. 36, 983–989 (1982).
Kostiala K.K.I., McGregor D.D., Logie P.S.: Tularemia in the rat. I. The cellular basis of the host resistance to infection.Immunology 28, 855–869 (1975).
Laemmli U.K.: Cleavage of structural proteins during the assembly of the heat of bacteriophage T4.Nature 227, 680–685 (1970).
Larson C.L., Bell J.F., Owen C.R.: The development of resistance in mice immunized with soluble antigen derived fromBacterium tularense.J. Immunol. 73, 221–225 (1954).
Macela A., Kovářová H., Kopecký J., Propper P.: Interaction ofFrancisella tularensis with the immunoregulatory system of mice, pp. 287–288 inBacteria and the Host (M. Rýc, J. Franěk, Eds.) Avicenum, Prague 1986.
Nutter J.E.: Antigens ofPasteurella tularensis: Preparative procedures.Appl. Microbiol. 22, 44–48 (1971).
Ormsbee R.A., Bell J.F., Larson C.L.: Studies ofBacterium tularense antigens. I. The isolation, purification, and biological activity of antigen preparation fromBacterium tularense.J. Immunol. 74, 351–358 (1955).
Ormsbee R.A., Larson C.L.: Studies onBacterium tularense antigens. II. Chemical and physical characteristics of protective antigen preparations.J. Immunol. 74, 359–370 (1955).
Rapp V.J., Ross R.F.: Antibody response of swine to outer membrane components ofHaemophilus pleuropneumoniae during infection.Infect. Immun. 54, 751–760 (1986).
Sandström G., Tärnvik A., Wolf-Watz H., Löfgren S.: Antigen fromFrancisella tularensis: Non-identity between determinants participating in cell-mediated and humoral reactions.Infect. Immun. 45, 101–106 (1984).
Sandström G., Tärnvik A., Wolf-Watz H.: Immunospecific T lymphocyte stimulation by membrane proteins fromFrancisella tularensis.J. Clin. Microbiol. 25, 641–644 (1987).
Towbin H., Staehelin T., Gordon J.: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some application.Proc. Nat. Acad. Sci. USA 76, 4350–4354 (1979).
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Stulik, J., Černa, J., Kovařova, H. et al. Protein heterogeneity ofFrancisella tularensis: Detection of proteins with antigenic determinants. Folia Microbiol 34, 316–323 (1989). https://doi.org/10.1007/BF02814474
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DOI: https://doi.org/10.1007/BF02814474