Abstract
Addition of glucose to a resting cell suspension of the yeastSaccharomyces cerevisiae was accompanied by marked shifts of the Gα-protein subunits from the plasma membrane to the cell interior. This process was rapid with half-times between <10 and 20 s. The decrease of the plasma membrane pool of the Giα/Goα- and Gqα/G11α-protein subunits correlated with an increase in acid-sensitive forms of these proteins which was recovered in the mitochondrial and/or lysosomal membrane fraction. In contrast to cells from higher organisms glucose-stimulated yeast exhibits an extremely rapid type of the redistribution (internalization). The question remains’open as to the functional significance of the internalized forms of the G-proteins as these remain sequestered from the plasma membrane. well after glucose has been consumed.
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Kotyk, A., Ihnatovych, I., Lapatiitis, G. et al. Subcellular shifts of trimeric G-proteins following activation of Baker’s yeast by glucose. Folia Microbiol 46, 391–396 (2001). https://doi.org/10.1007/BF02814427
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DOI: https://doi.org/10.1007/BF02814427