Abstract
Growth and extracellular polysaccharide production byPorphyridium cruentum were measured as a function of several culture parameters. Photon flux density of 75 μmol m−2 s−1 and CO2 concentration of 2.5% were found to be optimum for both growth and extracellular polysaccharide production. Interactive studies on these two parameters further confirmed that at these levels of photon flux density and CO2, when applied together, both growth (5.9·107 cells per mL) and extracellular polysaccharide production (1.9 g/L) were at the maximum. Maximum growth and extracellular polysaccharide production were observed at inoculum density of 106 cells per mL and aeration rate of 500 mL air per min per liter.
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Abbreviations
- EP:
-
extracellular polysaccharide
- PFD:
-
photon flux density
References
Anderson L.E.: Interaction between phytochemistry and activity of enzymes, pp. 271–281 inPhotosynthesis, II. Photosynthetic Carbon Metabolism and Related Processes (M. Gibbs, E. Latzko, Eds). Springer-Verlag, Berlin 1979.
Arad S., Adda M., Cohen E.: The potential of production of sulphate polysaccharides fromPorphyridium.Plant & Soil89, 117–127 (1985).
Arad S., Friedmam O., Rotem A.: Effect of nitrogen on polysaccharide production in aPorphyridium sp.Appl. Environ. Microbiol.54, 2411–2414 (1988).
Brody M., Vatter A.E.: Observations on cellular structures ofPorphyridium cruentum.J. Biophys. Biochem. Cytol.5, 289–299 (1959).
Clement-Metral J.D.: Preparation and some properties of protoplasts from red algaPorphyridium cruentum.J. Microsc. Biol. Cell26, 167–172 (1976).
Codd G.A., Stewart R.: Photoinactivation of ribulose bisphosphate carboxylase from green algae and cyanobacteria.FEMS Microbiol. Lett.8, 237–240 (1980).
Cohen Z., Arad S.: A closed system for outdoor cultivation ofPorphyridium.Biomass18, 59–67 (1989).
Dermoun D., Chaumont D., Thebault J., Dauta A.: Modelling of growth ofPorphyridium cruentum in connection with two interdependent factors: Light and temperature.Bioresource Technol.42, 113–117 (1992).
Gantt E., Lipschultz C.A.: Phycobilisomes ofPorphyridium cruentum.J. Cell Biol.54, 313–324 (1972).
Golueke C.G., Oswald W.J.: The mass culture ofPorphyridium cruentum.Appl. Microbiol.10, 102–107 (1962).
Gudin C., Chaumont D.: Cell fragility: the key problem of microalgae mass production in close photobioreactors.Bioresource Technol.38, 145–151 (1991).
Iqbal M., Grey D., Stepan-Sarkissian G.: Effect of nitrogen on growth, extracellular polysaccharide and intracellular phycoerythrin production by the unicellular red algaPorphyridium cruentum.Acta Microbiol. Pol.41, 65–73 (1992).
Iqbal M., Grey D., Stepan-Sarkissian G., Fowler M.W.: A flat-sided photobioreactor for culturing of microalgae.Aquacult. Eng.12, 183–190 (1993a).
Iqbal M., Grey D., Stepan-Sarkissian G., Fowler M.W.: Interactions between the unicellular red algaPorphyridium cruentum and associated bacteria.Eur. J. Phycol.28, 63–68 (1993b).
Jones R.F., Speer H.L., Kury W.: Studies on the growth of the red algaPorphyridium cruentum.Physiol. Plant.16, 636–643 (1963).
Lee Y.K., Tan H.M.: Effect of temperature, light intensity and dilution rate on the cellular composition of red algaPorphyridium cruentum in light chemostat cultures.MIRCEN J.4, 231–237 (1988).
Lee Y.K., Vonshak A.: The kinetics of photoinhibition and its recovery in the red algaPorphyridium cruentum Arch. Microbiol.150, 529–533 (1988).
Minkova K.M., Georgiev D.I., Houbavens-Ka N.B.: Light and temperature dependence of algal biomass and extracellular polysaccharide production fromP. cruentum.Biol. Physiol.40, 87–89 (1987).
Nielsen E.S.: Carbon dioxide concentration, respiration during photosynthesis and maximum quantum yield of photosynthesis.Plant Physiol.40, 87–89 (1953).
Ramus J.: Alcian Blue: A quantitative aqueous assay for algal acid and sulphated polysaccharides.J. Phycol.13, 345–348 (1977).
Řezanka T., Doucha J., Mareš P., Podojil M.: Effect of cultivation temperature and light intensity on fatty acid production in the red algaPorphyridium cruentum.J. Basic Microbiol.27, 275–278 (1987).
Richmond A.: Outdoor mass cultures of microalgae, pp. 285–330 inMicroalgal Mass Culture (A. Richmond, Ed.). CRC Press, Boca Raton (Florida) 1986.
Richmond A.: The challenge confronting industrial microagriculture: High photosynthetic efficiency in large-scale reactors.Hydrobiologia151/152, 117–121 (1987).
Savins, J.G.: Oil recovery process employing thickened aqueous driving fluid.US Pat. 4 079 544 (1978).
Sommerfeld M.R., Nichols H.W.: Comparative studies on the genusPorphyridium Naeg.J. Phycol.6, 67–78 (1970).
Sorokin C., Krauss R.W.: The dependence of cell division inChlorella on temperature and light intensity.Am. J. Bot.52, 331–339 (1965).
Tamiya H.: Mass culture of algae.Ann. Rev. Plant Physiol.18, 309–334 (1957).
Thepenier C., Gudin C.: Studies on optimal conditions for polysaccharide production byPorphyridium cruentum.MIRCEN J.1, 257–268 (1985).
Thepenier C., Gudin C., Thomas D.: Immobilization ofPorphyridium cruentum in polyurethane foam for the production of polysaccharide.Biomass7, 225–240 (1985).
Vonshak A., Cohen Z., Richmond A.: The feasibility of mass cultivation ofPorphyridium.Biomass8, 13–25 (1985).
Vonshak A., Abeliovich A., Bousibba S., Arad S., Richmond A.: Production ofSpirulina biomass: Effects of environmental factors and population density.Biomass2, 175–185 (1982).
von Witsch H., Bolze A., Hornung J.: Production of biomass and of extracellular polysaccharides in batch cultures ofPorphyridium aerugineum, Rhodophyceae.Ber. Dtsch. Bot. Ges.96, 469–481 (1983).
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Iqbal, M., Zafar, S.I. Effects of photon flux density, CO2, aeration rate, and inoculum density on growth and extracellular polysaccharide production byPorphyridium cruentum . Folia Microbiol 38, 509–514 (1993). https://doi.org/10.1007/BF02814405
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DOI: https://doi.org/10.1007/BF02814405